Collagen derived species-specific peptides for distinguishing donkey-hide gelatin(Asini Corii Colla)  

作　　者：Shuo Cai Ke-xuan Zhao Meng-tong Jiang Shu-ying Han Yun-feng Zheng Xun Liu Ming Zhao Jin-ao Duan Rui Liu 

机构地区：[1]National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing 210023,China [2]School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China [3]Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics,Nanjing University of Chinese Medicine,Nanjing 210023,China [4]Suzhou Vocational Health College,Suzhou 215009,China

出　　处：《Chinese Herbal Medicines》2021年第2期261-266,共6页中草药（英文版）

基　　金：funded by the National Natural Science Foundation of China (No.81973450);the National Key R&D Program of China (No.2018YFC1706100);the Jiangsu Qinglan Project;the Jiangsu “333” Project;the Young Researchers Training Project of China Association of Traditional Chinese Medicine (No.QNRC2C14);the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No.19KJB360020);the Open Project of Chinese Materia Medica First-Class Discipline of Nanjing University of Chinese Medicine (2020YLXK009)。

摘　　要：Objective:As an important food therapy product with traditional Chinese medicine(TCM) applications,donkey-hide gelatin(Asini Corii Colla,ACC) has been used for thousands of years.However,till now few effective strategy had been proposed to distinguish ACC from other animal hide gelatins,especially closely related horse-and mule-hide gelatins,which was an embarrassment of ACC quality control.Methods:Combined mass spectrometry and bioinformatic methods have been applied to identify and verify two ACC-specific peptides(Pep-1 and Pep-2) capable of distinguishing ACC from other closely related animal gelatins with high selectivity.Results:It confirmed that these two peptides could be not only used for distinguishing ACC from highly homologous horse-hide and mule-hide gelatins as well as other animal hide gelatins.Conclusion:The present study provides a simple method for species-specific peptides discovery,which can be used for assessing the quality of animal gelatin products,and ensure they are authenticable and traceable.

关 键 词：Asini Corii Colla Biomarkers COLLAGEN PEPTIDOMICS species-specific peptides 

分 类 号：R284[医药卫生—中药学]