光照颜色对虹鳟行为反应、血浆皮质醇和生化指标的影响  

Effects of light colors on behavior response,plasma cortisol and biochemical indexes of rainbow trout Oncorhynchus mykiss

作　　者：刘晓[1] 黄六一[1] 刘长东[1] 盛化香[1] 黄杰鸿 刘紫微 李玉岩[1] 任建钦 邬骞力 LIU Xiao;HUANG Liuyi;LIU Changdong;SHENG Huaxiang;HUANG Jiehong;LIU Ziwei;LI Yuyan;REN Jianqin;WU Qianli(College of Fisheries,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学水产学院,山东青岛266003

出　　处：《水产学报》2021年第5期740-747,共8页Journal of Fisheries of China

基　　金：国家重点研发计划(2019YFD0901003)。

摘　　要：光照是影响鱼类行为、生理以及生长的主要环境因子之一。为研究光照颜色对虹鳟行为反应、血浆皮质醇、生化指标的影响,实验根据鱼类行为学研究方法,分别将白、红、黄、蓝和绿5种光照颜色灯带铺设在室内试验水槽中央底部,将虹鳟放置在水槽同一侧,观察虹鳟在水槽中穿越灯带尾次数及行为反应;根据行为生理学研究方法,分析比较在不同光照颜色下虹鳟血浆皮质醇、总蛋白(TP)、白蛋白(ALB)、甘油三酯(TG)、总胆固醇(TC)、谷草转氨酶(AST)、谷丙转氨酶(ALT)的含量差异。结果显示:5种光照颜色下虹鳟穿越灯带尾次数均低于空白组,其中黄光、白光条件下穿越灯带尾次数均显著低于空白组和其余3种光照颜色,红光、蓝光、绿光和空白组之间无显著差异。黄光条件下血浆皮质醇浓度显著高于空白组和其余4种光照颜色,空白组和其余4种光照之间无显著差异。5种光照颜色下总蛋白(TP)、白蛋白(ALB)、甘油三酯(TG)、总胆固醇(TC)、谷草转氨酶(AST)、谷丙转氨酶(ALT)均具有显著差异。研究表明,虹鳟比较适应蓝光和绿光,对黄光和白光有一定的趋避反应,且黄光的趋避效果最强;虹鳟可通过自身生理调节以适应不同的光照颜色变化。In order to study the effect of light color on behavior response,plasma cortisol and blood biochemical indicators of O.mykiss,light stripes of white,red,yellow,blue and green,were respectively laid at the bottom of the tank center at a intensity of 56.9 lx.In each experiment,10 healthy and lively fish were placed on the same side of the tank to observe the times of O.mykiss crossing the light curtain in the tank.Each experiment lasted for 1 hour.The number of times of fish passing through the light curtain was observed and recorded every 10 minutes,no matter whether it was the first crossing or repeated crossing.Before each experiment,a control group was carried out,that is,a light stripe was laid without turning on the light,and then experiments were carried out in sequence under white light,red light,yellow light,blue light and green light.The experiment was repeated three times for each light color.Blood samples were collected after the fish were exposed to light for 1 hour.Plasma levels of cortisol,total protein(TP),albumin(ALB),triglyceride(TG),total cholesterol(TC),aspartame aminotransferase(AST)and alanine aminotransferase(ALT)under different light colors were determined by enzymelinked immune reaction kit(ELISA).The results showed as follows:(1)The number of times of O.mykiss crossing the light curtain under yellow and white light conditions was significantly lower than that of the control group and the other three light colors(P<0.05),there was no significant difference between red light,blue light,green light and control group(P>0.05).(2)The plasma cortisol concentration in yellow light condition was significantly higher than that in control group and the other four light colors(P<0.05),there was no significant difference between the control group and the other four light colors(P>0.05).(3)There were significant differences in TP,ALB,TG,TC,AST and ALT among five light colors(P<0.05).This study indicated that the O.mykiss was more adapted to blue light and green light,and had a certain avoidance reaction

关 键 词：虹鳟 光照颜色 鱼类行为 血浆皮质醇 生化指标 

分 类 号：S965[农业科学—水产养殖]