珠子参总皂苷对四氯化碳致大鼠肝纤维化的影响  

Effects of Total Saponins of Panax Japonicus on Hepatic Fibrosis Induced by Carbon Tetrachloride in Rats

作　　者：张丽 梁卓菲 张化为[2] 黄文丽 宋小妹[2] 宋蓓[3] ZHANG Li;LIANG Zhuofei;ZHANG Huawei;HUANG Wenli;SONG Xiaomei;SONG Bei(The First Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Shaanxi Xianyang 712000,China;Shaanxi University of Traditional Chinese Medicine,Shaanxi Xianyang 712046,China;The Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Shaanxi Xianyang 712046,China)

机构地区：[1]陕西中医药大学附属医院,陕西咸阳712000 [2]陕西中医药大学,陕西咸阳712046 [3]陕西中医药大学第二附属医院,陕西咸阳712046

出　　处：《陕西中医药大学学报》2021年第3期75-79,共5页Journal of Shaanxi University of Chinese Medicine

基　　金：陕西省科技厅重点研发计划项目(2018SF-324);陕西省科技厅计划项目(202400002);陕西中医药大学学科创新团队项目(2019-YL12)。

摘　　要：目的探讨珠子参总皂苷对四氯化碳(CCl_(4))诱导肝纤维化的预防保护作用。方法腹腔注射CCl_(4)制备肝纤维化模型,珠子参总皂苷灌胃给药进行干预,实验结束后,生化法和ELISA法检测各组大鼠肝功能及肝纤维化指标,HE和Massion染色法比较各组肝脏组织病理改变。结果珠子参总皂苷能显著降低肝纤维化大鼠血清AST、ALT、LN、HA和MDA水平,升高SOD和GSH-px水平,降低肝组织HYP水平。结论珠子参总皂苷具有一定的预防肝纤维化作用,其机制可能与抑制脂质过氧化、减少自由基的生成、提高组织的抗氧化能力等有关。Objective To investigate the protective effects of total saponins of Panax japonicus on hepatic fibrosis induced by carbon tetrachloride(CCl4). Methods Liver fibrosis model was established by intraperitoneal injection of CCl4. After the experiment, liver function and liver fibrosis indexes were detected by biochemical method and ELISA method, and pathological changes of liver tissue were compared by HE and Masson staining. Results Total saponins of Panax japonicus could significantly reduce the levels of AST, ALT, LN, HA and MDA in serum, increase the levels of SOD and GSH-px, and decrease the level of HYP in liver tissue. Conclusion Total saponins of Panax japonicus can prevent liver fibrosis to some extent, and its mechanism may be related to inhibiting lipid peroxidation, reducing the generation of free radicals and improving the antioxidant capacity of tissues.

关 键 词：珠子参总皂苷 肝纤维化 CCl_(4) 脂质过氧化 抗氧化 

分 类 号：R575.2[医药卫生—消化系统]