lncRNA PANDAR对KTC-3细胞生物学行为及RSK4信号通路的影响研究  

The effect of lncRNA PANDAR on the biological behavior of thyroid cancer KTC-3 cells and RSK4 signal pathway

作　　者：阿力木江·吾普尔 克力木·阿不都热依木[2] 玉素甫·买买提 艾尼·沙塔尔[1] Alimujiang Wupuer;Kelimu Abudureyimu;Yusufu Maimaiti;Aini Shataer(Department of Breast and Thyroid,Xinjiang Uygur Autonomous Region People's Hospital,Urumqi Xinjiang 830054,China;Minimally invasive,Hernia and abdominal Wall Surgery,Xinjiang Uygur Autonomous Region People's Hospital,Urumqi Xinjiang 830054,China)

机构地区：[1]新疆维吾尔自治区人民医院乳腺甲状腺科,新疆乌鲁木齐830054 [2]新疆维吾尔自治区人民医院微创、疝和腹壁外科,新疆乌鲁木齐830054

出　　处：《遵义医科大学学报》2021年第2期173-178,共6页Journal of Zunyi Medical University

基　　金：新疆维吾尔自治区自然科学基金计划项目(NO:2017D01C151)。

摘　　要：目的探究lncRNA PANDAR对甲状腺癌细胞(KTC-3细胞)生物学行为及RSK4信号通路的影响。方法在华中科技大学实验室购买KTC-3细胞,随机分为过表达组(O组):将lncRNA PANDAR慢病毒质粒与KTC-3细胞混合培养;阴性对照组(N组):将sh PANDAR慢病毒质粒与KTC-3细胞混合培养;空白对照组(B组):KTC-3细胞与慢病毒包装质粒混合培养。通过克隆实验检测KTC-3细胞增殖,免疫细胞化学法检测RSK4蛋白阳性表达,qRT-PCR法检测lncRNA PANDAR表达量,Transwell检测KTC-3细胞侵袭能力,Western blot法检测细胞中RSK4蛋白含量,Hoechst 33258荧光染色法检测细胞凋亡情况的差异性进行实验探究。结果与B组、N组比较,O组KTC-3细胞内lncRNA PANDAR表达量显著上升(P均<0.05),说明转染成功。与B组、N组相比较,O组KTC-3细胞增殖数量、侵袭能力显著升高(P均<0.05),B组与N组之间KTC-3细胞增殖数量、侵袭能力相差较小(P>0.05)。O组细胞的细胞核出现核固缩的概率显著高于B组、N组(P均<0.05),其中,B组、N组KTC-3细胞凋亡数量相差较小(P>0.05)。O组KTC-3细胞中RSK4蛋白表达、RSK4阳性数最高,与B组、N组相比较,O组KTC-3细胞RSK4蛋白表达、RSK4阳性数显著升高(P均<0.05),B组与N组之间KTC-3细胞中RSK4蛋白、RSK4阳性数含量相差较小(P>0.05)。结论lncRNA PANDAR过表达对KTC-3细胞生物行为有显著影响作用,对于细胞的增殖有正向促进能力,lncRNA PANDAR作用于甲状腺癌细胞生物行为可能与活化RSK4信号通路的表达相关。Objective Explore the effect of lncRNA PANDAR on the biological behavior of thyroid cancer cells(KTC-3 cells)and the expression of RSK4 signaling pathway.Methods Thyroid cancer KTC-3 cells were randomly divided into overexpression group(group O)by mixed culture of lncRNA PANDAR lentiviral plasmids with KTC-3 cells;negative control group(group N)by mixed culture of sh PANDAR lentiviral plasmid and KTC-3 cells;and blank control group(group B)bymixed culture of KTC-3 cells and lentiviral packaging plasmid.The proliferation of KTC-3 cells was detected by cloning experiment,the positive expression of RSK4 protein was detected by immunocytochemistry,the expression of lncRNA PANDAR was detected by qRT-PCR,the invasion ability of KTC-3 cells was detected by Transwell,the RSK4 protein content in the cells was detected by Western blot,Hoechst 33258 fluorescence staining was used to detect the difference of cell apoptosis for experimental exploration.Results Compared with group B and group N,the expression of lncRNA PANDAR in KTC-3 cells of group O increased significantly(all P<0.05),indicating that the transfection was successful.Compared with group B and group N,the number of KTC-3 cell proliferation and invasion ability in group O was significantly increased(all P<0.05),and the difference between group B and group N in the number of KTC-3 cell proliferation and invasion ability was small(P>0.05).The probability of nuclear pyknosis in the nucleus of the O group was significantly higher than that of the B and N groups(all P<0.05).Among them,the number of KTC-3 cell apoptosis in the B and N groups was relatively small(P>0.05).RSK4 protein expression and RSK4 positive number were the highest in KTC-3 cells of O group.Compared with group B and N,RSK4 protein expression and RSK4 positive number of KTC-3 cells of O group were significantly increased(all P<0.05)The difference in the number of RSK4 protein and RSK4 positive number in KTC-3 cells between group B and N was small(P>0.05).Conclusion Overexpression of lncRNA PANDAR ha

关 键 词：lncRNA PANDAR SK4信号通路 甲状腺癌 生物学行为 

分 类 号：R736.1[医药卫生—肿瘤]