龙胆酵素的发酵工艺优化及其生化功效研究  

Optimization of fermentation process for gentian ferment and analysis of its biochemical efficacy

作　　者：陶宏兵 杨娟[1] 邓小锋 叶子翠 施庆珊 谢小保[2] 黄小茉[1] TAO Hongbing;YANG Juan;DENG Xiaofeng;YE Zicui;SHI Qingshan;XIE Xiaobao;HUANG Xiaomo(Guangdong Demay New Materials Technology Co. Ltd., Zhaoqing, Guangdong 526238, China;Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, Guangdong 510070, China)

机构地区：[1]广东迪美新材料科技有限公司,广东肇庆526238 [2]广东省微生物研究所省部共建华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室,广东广州510070

出　　处：《工业微生物》2021年第3期27-30,共4页Industrial Microbiology

基　　金：肇庆市科技计划项目(项目编号2019G006);肇庆市第三批西江创新团队项目(肇人才办【2019】5号)。

摘　　要：以龙胆草为发酵底物,龙胆苦苷为评价指标,采用正交实验优化龙胆酵素的发酵工艺,用高效液相色谱(HPLC)分析龙胆酵素中龙胆苦苷含量,并评估其生化功效。结果表明,最优龙胆酵素发酵工艺为菌株组合酿酒酵母加植物乳杆菌1∶1、接种量10%、发酵温度30℃、发酵时间72 h。在此优化工艺条件下,发酵液龙胆苦苷含量达到(1823±21)μg/mL,其透明质酸酶抑制率、DPPH、·OH和O^(-)_(2)·自由基的清除能力分别达到89.2%、77.5%、70.2%和74.1%,表明该工艺的发酵液具有很好的抗敏和自由基清除功效。Taking gentian as fermentation substrate and gentiopicroside as index,the fermentation process of gentian ferment was optimized by single factor and orthogonal experiments,and the gentiopicroside content in gentian ferment was analyzed by HPLC,meanwhile its biochemical efficacy was evaluated.The results showed that the optimized fermentation process of the gentian ferment were as follows:compound strain Saccharomyces cerevisiae and Lactobacillus plantarum ratio 1∶1,inoculum size 10%,fermentation temperature 30℃and fermentation time 48 h.Under the optimal conditions,the gentiopicroside content of the gentian ferment was(1823±25)μg/mL.The fermentation liquor had good anti-allergic and free radicals clearance.The hyaluronic acid enzyme inhibition rate,DPPH,·OH and O^(-)_(2) radicals clearance rates were 89.2%,77.5%,70.2%and 74.1%,respectively.

关 键 词：龙胆酵素 发酵 龙胆苦苷 生化功效 

分 类 号：R28[医药卫生—中药学]