不同麻醉方式对自发性高血压大鼠脑出血凋亡基因表达水平的影响  

Effects of Different Anesthesia Methods on the Expression of Apoptotic Genein Spontaneous Hypertensive Rats with Intracerebral Hemorrhage

作　　者：赵广平[1] 陈永学[1] 甄书青[1] 侯俊德[1] 刘飞[1] ZHAO Guangping;CHEN Yongxue;ZHEN Shuqing(Handan Central Hospital, Hebei Handan 056001, China)

机构地区：[1]河北省邯郸市中心医院麻醉科,河北邯郸056001

出　　处：《河北医学》2021年第6期881-886,共6页Hebei Medicine

基　　金：河北省重点科技研究项目,(编号:20200480)。

摘　　要：目的:探究分析不同麻醉方式对自发性高血压大鼠脑出血模型神经细胞凋亡及相关蛋白表达水平的影响。方法:30只自发性高血压大鼠,均采用大鼠自体血注入法构建脑出血模型。采用随机数字表法随机分为麻醉A组及麻醉B组,每组15只,A组大鼠给予静脉吸入复合麻醉,B组大鼠给予丙泊酚复合舒芬太尼靶控静脉麻醉。观察比较不同组别大鼠麻醉建模后6h、12h、24h、48h、72h时神经行为学评分、脑组织含水量差异,采用TUNEL法检测不同时点脑组织细胞凋亡情况,采用免疫组化实验检测脑出血周围脑组织Bax、Bcl-2、Caspase-3等凋亡相关蛋白表达情况,同时采用RT-PCR实验和Western Blot实验检测术后即刻脑出血周围脑组织Bax、Bcl-2、Caspase-3等凋亡相关蛋白就基因表达情况。结果:脑出血术后6h大鼠开始出现不同程度神经行为学改变,随着时间点的延长,出血区周围脑组织含水量明显增加,凋亡细胞数明显增多,凋亡相关蛋白Bax、Bcl-2及Caspase-3表达量也明显增加,与麻醉A组比较,麻醉B组大鼠脑出血术后6h、12h、24h、48h、72h各时点神经行为学评分及缺血区脑组织含水量均明显较低(P<0.05),细胞凋亡数明显较少(P<0.05),凋亡相关蛋白Bax及Caspase-3 mRNA及蛋白表达水平均明显低于同时点麻醉A中组水平(P<0.05),凋亡抑制蛋白Bcl-2mRNA及蛋白表达水平则明显高于同时点麻醉A中组水平(P<0.05)。结论:在自体血注入法构建的脑出血动物模型中,采用丙泊酚复合舒芬太尼靶控静脉麻醉能减少脑出血后凋亡相关蛋白表达,减少神经细胞凋亡,减轻脑水肿程度,改善神经行为学评分,在继发性脑出血损伤中发挥保护作用。Objective:To explore the effects of different anesthesia methods on neuronal apoptosis and expression of related proteins in spontaneous hypertensive rats with intracerebral hemorrhage.Methods:30 spontaneously hypertensive rats were used to establish cerebral hemorrhage models by autologous blood injection.They were randomly divided into anesthesia group A and anesthesia group B with 15 rats in each group.Rats in group A were given intravenous inhalation combined with anesthesia,while rats in group B were given target controlled intravenous anesthesia with propofol combined with sufentanil.After the anesthesia took effect,the rats in both groups were injected with autologous blood to build cerebral hemorrhage models.The neurobehavioral scores and brain water content of both groups of rats were observed and compared at 6h,12h,24h,48h and 72h after anesthesia modeling.TUNEL method was used to detect the apoptosis of brain tissue at different time points.The expression of Bax,Bcl-2,caspase-3 and other apoptosis related proteins in brain tissue around intracerebral hemorrhage were detected by immunohistochemistry.The expression of Bax,Bcl-2,caspase-3 and other apoptosis related proteins in brain tissue around intracerebral hemorrhage were detected by RT-PCR and Western blot.Results:Neurobehavioral changes began to appear at 6 hours after intracerebral hemorrhage operation.With the extension of time point,the water content of brain tissue around the hemorrhage area increased significantly,the number of apoptotic cells increased significantly,and the expression of apoptosis related proteins Bax,Bcl-2 and caspase-3 also increased significantly.Compared with anesthesia group A,the nerve of anesthesia group B at 6 h,12 h,24 h,48 h,72 h after operation was significantly increased.The expression levels of Bax and caspase-3 mRNA and protein were significantly lower than those of group A at the same time point(P<0.05),while the expression of bcl-2 mRNA and protein was significantly higher than that of group A at the same time

关 键 词：自发性高血压大鼠 脑出血 细胞凋亡 靶控静脉麻醉 

分 类 号：R74[医药卫生—神经病学与精神病学]