膀胱癌病理分级与PGAP2基因表达的相关性及分子生物学关系的研究  

Study on the relationship between PGAP2 gene expression and pathological grade of bladder cancer and molecular biology

作　　者：刘玉錾 宋颖刚 高昆[1] 高双友[2] LIU Yuzan;SONG Yinggang;GAO Kun;GAO Shuangyou(Department of Urology,Linxi Hospital of Kailuan General Hospital,Tangshan,Hebei,063100,China;Department of Urology,Kailuan General Hospital)

机构地区：[1]开滦总医院林西医院泌尿外科,河北唐山063100 [2]开滦总医院泌尿外科

出　　处：《临床泌尿外科杂志》2021年第5期377-381,共5页Journal of Clinical Urology

摘　　要：目的:检测不同病理分级膀胱癌患者组织PGAP2表达水平,并探讨其表达水平与膀胱癌病理分级及分子生物学关系。方法:选取2011年8月—2015年4月于开滦总医院林西医院住院的78例膀胱癌患者肿瘤组织作为肿瘤组,膀胱癌患者均为尿路上皮癌,组织学分级按照WHO分级标准:Ⅰ级24例、Ⅱ级29例、Ⅲ级25例;根据膀胱癌患者肿瘤组织PGAP2 mRNA水平平均值,将患者分为PGAP2高表达组(n=39)和PGAP2低表达组(n=39);根据患者出院后5年内是否出现复发或因膀胱癌死亡,将患者分为预后良好46例和预后不良32例。选取膀胱癌患者相应癌旁正常组织作为正常组。采用实时荧光定量PCR(qRT-PCR)法检测膀胱癌肿瘤组织及癌旁正常组织PGAP2 mRNA水平;Ualcan数据库检索验证PGAP2在正常膀胱组织及膀胱癌肿瘤组织中的表达;分析肿瘤组织PGAP2 mRNA水平与膀胱癌患者病理分级等临床病理特征的关系;多因素Cox回归分析影响膀胱癌预后的因素。结果:肿瘤组PGAP2 mRNA水平高于正常组,差异有统计学意义(P<0.05),与Ualcan数据库结果一致;PGAP2高表达组肿瘤多发、组织浸润深度为肌层浸润性膀胱癌、预后不良的患者比例均高于PGAP2低表达组,差异有统计学意义(P<0.05);肿瘤组织PGAP2表达水平与膀胱癌病理组织学分级有关,差异有统计学意义(P<0.05);病理分级、PGAP2是影响膀胱癌预后的独立危险因素(P<0.05)。结论:膀胱癌患者肿瘤组织PGAP2表达水平与病理分级等临床病理特征密切相关,且是影响膀胱癌预后的独立危险因素,可对膀胱癌的病情评价及治疗提供一定理论依据。Objective:To detect the expression level of PGAP2 in different pathological grades of bladder cancer,and to explore the relationship between the expression level and pathological grades and molecular biology of bladder cancer.Methods:From August 2011 to April 2015,bladder cancer tissues from 78 patients in Linxi Hospital of Kailuan General Hospital were selected as tumor group,and all bladder cancer patients were urothelial cancer.According to WHO classification standard,the histological classification was:24 cases of grade Ⅰ,29 cases of grade Ⅱ,25 cases of grade Ⅲ.According to the average level of PGAP2 mRNA in bladder cancer,the patients were divided into two groups:PGAP2 high expression group(n=39)and PGAP2 low expression group(n=39).According to the recurrence or death of bladder cancer within 5 years after discharge,the patients were divided into 46 with good prognosis and 32 with poor prognosis.The normal tissues adjacent to the bladder cancer were selected as the normal group.The level of PGAP2 mRNA in tumor tissue of bladder cancer and normal tissues adjacent to the bladder cancer were detected by real-time fluorescence quantitative PCR(qRT-PCR),and the expression of PGAP2 in normal bladder tissue and bladder cancer tissue were verified by the search of Ualcan database.The relationship between PGAP2 mRNA level in tumor tissue and clinicopathological characteristics of bladder cancer patients was analyzed,and multivariate Cox regression was used to analyze the prognostic factors of bladder cancer.Results:The level of PGAP2 mRNA in tumor group was higher than that in normal group.The difference was statistically significant(P<0.05),which was consistent with the result of Ualcan database.The proportions of patients with multiple tumors,infiltration depth of muscle layer invasive bladder cancer and poor prognosis in PGAP2 high expression group were higher than those in PGAP2 low expression group,and the difference was statistically significant(P<0.05).The expression level of PGAP2 was related to the his

关 键 词：膀胱癌 病理分级 肿瘤组织 PGAP2基因 

分 类 号：R737.14[医药卫生—肿瘤]