纳豆冻干粉对2型糖尿病碱性磷酸酶与炎症反应的影响  

Effect of natto freeze-dried powder on alkaline phosphatase andinflammation in type 2 diabetes

作　　者：王语聪 谢智鑫 张学艳[3] 王英男 韩建春[1,2] WANG Yucong;XIE Zhixin;ZHANG Xueyan;WANG Yingnan;HAN Jianchun(College of Food Science,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Green Food Science Research Institute,Harbin 150028,China;Northeast Agricultural University Hospital,Harbin 150030,China)

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]黑龙江省绿色食品科学研究院,黑龙江哈尔滨150028 [3]东北农业大学医院,黑龙江哈尔滨150030

出　　处：《食品与发酵工业》2021年第11期68-74,共7页Food and Fermentation Industries

基　　金：黑龙江省绿色食品科学研究院横向课题(SKY20200921-1)。

摘　　要：为探究纳豆冻干粉对2型糖尿病碱性磷酸酶、细菌内毒素和肠道中炎性因子的影响,采用高糖高脂饮食+链脲佐菌素腹腔注射建立2型糖尿病模型,连续给予大鼠灌胃纳豆冻干粉水溶液(4、8 g/kg),测定大鼠体质量及血糖变化、粪便中肠道碱性磷酸酶(intestinal alkaline phosphatase,IAP)的活力及脂多糖(lipopolysaccharides,LPS)的浓度变化,并用Meso Scale Discovery(MSD)多因子方法检测肠道中炎症因子[白细胞介素-6.IL-6)、肿瘤坏死因子-α(TNF-α)、γ干扰素(IFN-γ)、白细胞介素-1.IL-1β)、白细胞介素-4.IL-4)、白细胞介素-5.IL-5)、白细胞介素-10(IL-10)、白细胞介素-13.IL-13)、生长调节致癌基因α(KC/GRO)]浓度变化。结果表明,与模型对照组相比,纳豆冻干粉剂量组空腹血糖浓度显著降低(P<0.05),IAP活力显著增加(P<0.05),LPS浓度显著降低(P<0.05),且肠液中促炎因子IL-6、TNF-α、IFN-γ、IL-1β的浓度显著减少(P<0.05),而抗炎因子IL-4、IL-5、IL-10、IL-13的浓度显著增加(P<0.05),趋化因子KC/GRO的浓度显著下降(P<0.05)。因此纳豆冻干粉可以促进肠碱性磷酸酶活性,并可有效调节2型糖尿病小鼠血糖及炎症反应。To investigate the effects of natto lyophilized powder on alkaline phosphatase,bacterial endotoxin and intestinal inflammatory factors in type 2 diabetes mellitus(T2DM),the type 2 diabetic model was established by intraperitoneal injection of high sugar diet and streptozotocin.The changes of body weight and blood glucose,the activity of intestinal alkaline phosphatase(IAP)in feces and the concentration of bacterial endotoxin were measured,and the concentrations of inflammatory factors(interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),interferon gamma(IFN-γ),interleukin-1(IL-1β),interleukin-4(IL-4),interleukin-5(IL-5),interleukin-10(IL-10),and interleukin-13(IL-13)growth regulatory oncogeneα(KC/GRO))in the intestine were analyzed by MSD multifactor method.The result showed that compared with the model control group,the fasting blood glucose concentration significantly decreased(P<0.05),the IAP activity increased significantly(P<0.05),the concentration of bacterial endotoxin decreased significantly(P<0.05),and the concentrations of proinflammatory cytokines IL-6,TNF-α,IFN-γ,and IL-1βin the intestinal fluid were significantly reduced(P<0.05),while the concentrations of cytokines IL-4,IL-5,IL-10,and IL-13 increased significantly(P<0.05),and the concentration of chemokine KC/GRO decreased significantly(P<0.05)in the natto lyophilized powder dose group.Therefore,natto lyophilized powder can promote the activity of intestinal alkaline phosphatase,and effectively regulate the blood glucose and inflammatory response in T2DM mice.

关 键 词：炎症 糖尿病 纳豆冻干粉 炎症因子 肠碱性磷酸酶 

分 类 号：TS201.4[轻工技术与工程—食品科学]