Qualitation of Chuanxiong Rhizome essential oil and determination of 3-butylidenephthalide and ligustilide by GC-MS  

川芎挥发油的GC-MS定性分析及丁烯基苯酞和藁本内酯的含量测定

作　　者：Ximei Yan Taixiang Gao Liyao Shi Rui Wang 闫玺镁;高太祥;石莉尧;王瑞(山西中医药大学中药与食品工程学院,山西晋中030619)

机构地区：[1]School of Traditional Chinese Materia Medical,Shanxi University of Traditional Chinese Medicine,Jinzhong 030619,China

出　　处：《Journal of Chinese Pharmaceutical Sciences》2021年第5期434-445,共12页中国药学（英文版）

基　　金：Shanxi pharmaceutical comprehensive development and utilization collaborative innovation center project(Grant No.2017JYXT15);Science and technology innovation team project of Shanxi University of Traditional Chinese Medicine(Grant No.2018TD-008);“Basic research project”of Shanxi University of traditional Chinese Medicine(Grant No.2020PY-JC-20)。

摘　　要：Chuanxiong Rhizoma(rhizomes of Ligusticumchuanxiong Hort)is a classical Chinese medicine,and it is usually used for the treatment of cardiovascular diseases.The essential oil is the main active constituent of Chuanxiong.In this study,a GC-MS quantitative method based on the SIM scanning mode and batch processing analysis was established,and the total contents of 3-butylidenephthalide and ligustilide were used as the indicators to optimize three extraction methods,including hydro-distillation extraction(HDE),ultrasound-assisted extraction(UAE)and supercritical fluid extraction(SFE).Then the volatile oil obtained by three extraction methods was analyzed qualitatively.The results showed that the volatile oil obtained by the UAE had the highest contents of 3-butylidenephthalide and ligustilide,the volatile oil extracted by HDE had the most types of chemical components,and the SFE volatile oil had the highest oil yield.川芎是一种治疗心血管疾病的传统中药,其主要活性成分为挥发油。本研究建立了一种基于SIM扫描模式和批处理分析的GC-MS定量方法,并以丁烯基苯酞和藁本内酯的总含量为指标,对水蒸气蒸馏法、超声提取法和CO_(2)超临界萃取法三种挥发油提取方法进行工艺优化,对三种方法所得挥发油进行成分鉴定和含量测定。结果表明,3种方法提取的挥发油中,超声提取所得丁烯基苯酞和藁本内酯的提取率最高,水蒸气蒸馏法所得挥发油的化学成分种类最多,超临界萃取法所得挥发油量最多,得油率最高。

关 键 词：Ligusticumchuanxiong Hort 3-Butylidenephthalide LIGUSTILIDE GC-MS SIM scanning 

分 类 号：R917[医药卫生—药物分析学]