PHF19对宫颈癌细胞增殖、侵袭和迁移的影响  

Effect of PHF19 on proliferation,invasion and migration of cervical cancer cells

作　　者：刘瑞芳 张志迪 陈梦茜[1] 金在顺[1] LIU Rui-fang(Department of Pathology, Mudanjiang Medical University, Mudanjiang 157011, China)

机构地区：[1]牡丹江医学院基础医学院病理学与病理生理学教研室,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2021年第3期8-12,共5页Journal of Mudanjiang Medical University

基　　金：黑龙江省省属高等学校基本科研业务费项目(2018-KYYWFMY-0008);牡丹江市科学技术计划项目(Z2017s0050)。

摘　　要：目的探讨PHF19基因对宫颈癌细胞株Hela和HCC94的增殖、侵袭和迁移的影响。方法培养人宫颈癌Hela细胞和HCC94细胞,用siRNA敲低PHF19基因的表达。RT-qPCR检测不同组细胞中PHF19基因mRNA水平的表达情况。采用CCK-8检测细胞的增殖情况。采用细胞划痕、Transwell实验观察PHF19对宫颈癌细胞迁移、侵袭能力的影响。结果RT-qPCR结果显示,与对照组Hela细胞和HCC94细胞相比,siRNA-2-50组中PHF19的mRNA表达水平减少(P<0.05);CCK-8结果表明,与对照组Hela细胞和HCC94细胞相比,siRNA-2-50组细胞增殖活性降低(P<0.05);划痕和Transwell实验证实了,与对照组相比,siRNA-2-50组细胞侵袭和迁移能力降低(P<0.05)。结论PHF19基因可能促进宫颈癌Hela细胞和HCC94细胞的增殖和侵袭迁移能力。Objective To investigate the effect of PHF19 gene on proliferation,invasion and migration of cervical cancer cell lines Hela and HCC94.Methods Human cervical cancer Hela and HCC94 cells were cultured,and the expression of PHF19 gene was knocked down by siRNA.RT-qPCR was used to detect the expression of PHF19 mRNA in different groups.CCK-8 was used to detect cell proliferation.Wound-Healing and Transwell assay were used to observe the effects of PHF19 on the migration and invasion of cervical cancer cells.Results RT-qPCR results showed that the expression of PHF19 mRNA in HeLa and HCC94 cells decreased after siRNA transfection(P<0.05).CCK-8 results showed that the proliferation activity of HeLa and HCC94 cells decreased after siRNA transfection(P<0.05).Wound and Transwell experiments confirmed that compared with the control group,the invasion and migration ability of HeLa and HCC94 cells decreased after siRNA transfection(P<0.05).Conclusion PHF19 gene may promote the proliferation,invasion and migration of Hela and HCC94 cells.

关 键 词：宫颈癌 PHF19基因 侵袭迁移 增殖 

分 类 号：R737.33[医药卫生—肿瘤]