白蚁肠道具耐高温耐碱β-葡萄糖苷酶活性共生菌株的筛选鉴定及其产酶条件优化  

Screening and Identification of Bacteria from Termite Producingβ-Glucosidase and Optimization of Conditions for Enzyme Production

作　　者：赵素雅 刘苏瑶 魏旭阳 尹晓燕 牛秋红[1] ZHAO Su-ya;LIU Su-yao;WEI Xu-yang;YIN Xiao-yan;NIU Qiu-hong(College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang Henan 473061, China)

机构地区：[1]南阳师范学院生命科学与农业工程学院,河南南阳473061

出　　处：《西南师范大学学报（自然科学版）》2021年第6期65-74,共10页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金面上项目(31570120);河南省能源微生物资源可持续利用创新型科技团队项目.

摘　　要：从低等木食性白蚁——黑胸散白蚁肠道中通过七叶苷与柠檬酸铁铵的显色反应筛选出14株产β-葡萄糖苷酶的菌株.采用选择培养复筛出1株在60℃,pH值为8的培养条件下,具有耐高温耐碱性的产β-葡萄糖苷酶的菌株BH1.通过对BH1菌株进行形态学鉴定,结合16S rRNA序列对比分析,初步判断BH1属于芽孢杆菌属(Bacillus sp.).运用单因素和正交试验法对其在产酶过程中的培养条件,如时间、温度、pH等进行优化.通过单因素试验可知BH1菌株产酶最佳碳源是麦麸,氮源是酵母膏,无机盐是NaCl,最适pH值为8.0,最适温度为60℃.BH1菌株最佳酶活力为36.24 U/mL,在pH值为9.0,温度为70℃时还具有相对较高的酶活,也进一步说明了该菌株所产的β-葡萄糖苷酶具有耐高温耐碱的特性.Fourteen strains ofβ-glucosidase-producing bacteria were screened from the intestinal tract of the lower cassava termite,Black-breasted Termite,by color reaction of escin and ferric ammonium citrate.With selective culture,a strain named BH1 producingβ-glucosidase with high temperature and alkali tolerance under 60℃and pH=8 culture conditions was selected.Through morphological identification,combined with 16S ribosomal RNA identification sequence analysis,the BH1 strain was preliminarily identified to belong to Bacillus sp.The single factor and orthogonal test method were used to optimize the cultivation conditions,such as time and temperature,in the process of producing enzymes.Through a single factor test,it can be seen that the best carbon source for enzyme production of BH1 strain is wheat bran,nitrogen source is yeast extract,inorganic salt is NaCl,the optimum pH is 8.0,and the optimum temperature is 60℃.The optimal enzyme activity of BH1 strain is 36.24 U/mL.It also has a relatively high enzyme activity at a pH of 9.0 and a temperature of 70℃,which further demonstrates that theβ-glucosidase produced by this strain has high temperature and alkali resistance.

关 键 词：黑胸散白蚁 Β-葡萄糖苷酶 筛选鉴定 耐高温耐碱 

分 类 号：Q939.9[生物学—微生物学]