塞来昔布对幽门螺杆菌感染的胃癌细胞凋亡及炎症反应的影响  

Effect of Celecoxib on apoptosis and inflammatory response of gastric cancer cells infected with Helicobacter pylori

作　　者：王竞[1] 段志英[1] 杨明月 朱秀芳[1] 霍晓辉[1] 范红云[1] WANG Jing;DUAN Zhiying;YANG Mingyue;ZHU Xiufang;HUO Xiaohui;FAN Hongyun(Department of Gastroenterology,the First Hospital of Hebei Medical University,Shijiazhuang 050081,China)

机构地区：[1]河北医科大学第一医院消化内科,河北石家庄050081

出　　处：《胃肠病学和肝病学杂志》2021年第6期617-621,共5页Chinese Journal of Gastroenterology and Hepatology

基　　金：河北省卫生厅科研基金项目(20180218)。

摘　　要：目的探讨塞来昔布对幽门螺杆菌(Helicobacter pylori,H.pylori)感染的胃癌细胞凋亡及炎症反应的影响及机制。方法以终浓度为50、75、100μmol/L的塞来昔布作用于H.pylori感染的人胃癌SGC-7901细胞,MTT法检测作用24、48和72 h的细胞增殖;作用48 h,流式细胞术检测细胞凋亡率,Western blotting检测PCNA、Bcl-2、Bax和p-AKT3蛋白表达,qRT-PCR检测IL-6、IL-8和miR-145表达。双荧光素酶报告基因实验检测miR-145和AKT3的靶向关系。结果不同浓度塞来昔布均可明显抑制SGC-7901细胞增殖,且呈剂量、时间依赖性(P<0.05)。不同浓度塞来昔布均可明显促进细胞凋亡,下调PCNA、Bcl-2、p-AKT3、IL-6和IL-8表达,上调Bax和miR-145表达,呈剂量依赖性(P<0.05)。双荧光素酶报告基因实验结果显示,miR-145和AKT3存在靶向关系。结论塞来昔布可抑制H.pylori感染的胃癌细胞增殖,促进细胞凋亡,降低炎症因子IL-6和IL-8表达,机制可能与塞来昔布引起miR-145表达改变进而调控其靶基因AKT3表达有关。Objective To investigate the effect and mechanism of Celecoxib on apoptosis and inflammatory response of gastric cancer cells infected with Helicobacter pylori(H.pylori).Methods Human gastric cancer SGC-7901 cells were treated with Celecoxib(50,75,100μmol/L).The proliferation of SGC-7901 cells was detected by MTT assay at 24 hours,48 hours and 72 hours.Cells were treated for 48 hours,the apoptosis rate was detected by flow cytometry,the protein expression of PCNA,Bcl-2,Bax and p-AKT3 was detected by Western blotting,and the expression of IL-6,IL-8 and miR-145 was detected by qRT-PCR.Double luciferase reporter gene assay was used to detect the targeting relationship between miR-145 and AKT3.Results Different concentrations of Celecoxib could significantly inhibit the proliferation of SGC-7901 cells in a dose-and time-dependent manner(P<0.05).Different concentrations of Celecoxib could significantly promote apoptosis,down regulate the expression of PCNA,Bcl-2,p-AKT3,IL-6 and IL-8,and up regulate the expression of Bax and miR-145 in a dose-dependent manner(P<0.05).Double luciferase reporter gene assay showed that there was targeting relationship between miR-145 and AKT3.Conclusion Celecoxib can inhibit the proliferation of H.pylori-infected gastric cancer cells,promote cell apoptosis,and reduce the expression of inflammatory factors IL-6 and IL-8.The mechanism may be related to the change of miR-145 expression caused by Celecoxib to regulate its target genes AKT3 expression.

关 键 词：胃癌 塞来昔布 幽门螺杆菌 凋亡 炎症反应 

分 类 号：R735.2[医药卫生—肿瘤]