异甘草酸镁通过ERK1/2信号通路对大鼠急性肝衰竭保护机制的初步研究  

Experimental study of magnesium isoglycyrrhizinate promoting the prognosis of acute liver failure rats through ERK1/2 pathway

作　　者：刘亨晶 李初谊 王盼 李斌 王俊科[1] 郑英 卢利霞 张久聪 于晓辉 甄英丽 LIU Hengjing;LI Chuyi;WANG Pan;LI Bin;WANG Junke;ZHENG Ying;LU Lixia;ZHANG Jiucong;YU Xiaohui;ZHEN Yingli(Department of Gastroenterology,the 940th Hospital of Joint Logistic Support Force of People's Liberation Army,Lanzhou 730050;Gansu University of Traditional Chinese Medicine;Department of First Resident Outpatient,the 940th Hospital of Joint Logistic Support Force of People's Liberation Army,China)

机构地区：[1]中国人民解放军联勤保障部队第九四〇医院消化内科,甘肃兰州730050 [2]甘肃中医药大学 [3]中国人民解放军联勤保障部队第九四医院第一驻派门诊部

出　　处：《胃肠病学和肝病学杂志》2021年第6期671-675,共5页Chinese Journal of Gastroenterology and Hepatology

基　　金：甘肃省科技支撑项目(1604FKCA101);西北民族大学中央高校基本科研业务费资助(31920200014)。

摘　　要：目的探讨异甘草酸镁(MgIG)通过ERK1/2信号通路对急性肝衰竭(acute liver failure,ALF)大鼠肝功能的转归作用,深入揭示MgIG的保肝机制。方法将60只Wistar大鼠随机分为5组,其中12只为正常对照组(给予清洁饮用水),其余48只为实验组,均制作成ALF模型,分为模型组(0.9%的生理盐液)和MgIG治疗组(25 mg/kg、50 mg/kg和100 mg/kg)。48 h后取大鼠肝脏组织同时观察病理学变化,Western blotting法检测每组大鼠肝组织中ERK1/2和pERK1/2的表达,qRT-PCR法检测各组大鼠肝组织中ERK1/2 mRNA的含量。结果实验组大鼠肝组织中ERK1/2蛋白表达与正常对照组比较,差异无统计学意义(P>0.05);模型组ALF大鼠肝脏组织中pERK1/2、ERK1/2 mRNA的表达明显高于正常对照组(P<0.01),治疗组大鼠肝组织中pERK1/2、ERK1/2 mRNA的表达随着MgIG浓度的增加逐渐下调(P<0.01),尤其在MgIG 100 mg/kg组下调最为显著。结论ALF的发生发展与ERK1/2信号通路的磷酸化密切相关,MgIG可能通过下调ERK1/2的表达发挥其抗炎保肝作用。Objective To explore the effect of magnesium isoglycyrrhizinate(MgIG)on liver function in rats with acute liver failure(ALF)through ERK1/2 signaling pathway,and to further reveal the protective mechanism of MgIG.Methods Sixty Wistar rats were randomly divided into 5 groups,12 rats of which were normal control group(giving clean drinking water)and the other 48 rats were experimental groups,all of which were made into ALF model and divided into model group(0.9%NS)and MgIG treatment groups(25 mg/kg,50 mg/kg and 100 mg/kg).After 48 hours,the liver of each groups was taken out and the pathological changes of liver tissue were observed,the expressions of ERK1/2 and pERK1/2 were detected by Western blotting and the expression level of ERK1/2 mRNA was tested by qRT-PCR.Results There was no significant difference in the expression of ERK1/2 protein between the experimental group and the normal control group(P>0.05).The expression of pERK1/2 and ERK1/2 mRNA in the model group was significantly higher than that in the normal control group(P<0.01).The expression of pERK1/2 and ERK1/2 mRNA decreased with the increase of MgIG concentration(P<0.01),especially in the MgIG 100 mg/kg group.Conclusion The occurrence and progress of ALF are closely related to the phosphorylation of ERK1/2 protein,and the anti-inflammatory and hepatoprotective effects of MgIG may be achieved by down-regulating ERK1/2 signaling pathway.

关 键 词：异甘草酸镁 ERK1/2信号通路 急性肝衰竭 

分 类 号：R575.3[医药卫生—消化系统]