lncRNA PWRN2在胶质瘤组织中的表达及对胶质瘤细胞增殖和迁移能力的影响  

Expression of lncRNA PWRN2 in glioma tissue and its influence on glioma cell proliferation and migration

作　　者：马迎辉[1] 胡胜[1] 胡骁 吴勇[1] 金杰[1] Ma Yinghui;Hu Sheng;Hu Xiao;Wu Yong;Jin Jie(Department of Neurosurgery,Huangshi Central Hospital,Edong Healthcare Group(Hospital Affiliated to Hubei Polytechnic University),Huangshi 435000,China)

机构地区：[1]鄂东医院集团黄石市中心医院湖北理工学院附属医院神经外科,435000

出　　处：《国际医药卫生导报》2021年第11期1680-1683,共4页International Medicine and Health Guidance News

摘　　要：目的观察长链非编码RNA(long-chainnoncodingRNA,lncRNA)PWRN2在胶质瘤组织中的表达及对胶质瘤细胞增殖和迁移能力的影响,探讨PWRN2可能的作用机制。方法运用实时荧光定量聚合酶链式反应(qPCR)技术检测PWRN2在42例胶质瘤组织及对应癌旁组织、5种胶质瘤细胞株及正常脑胶质细胞中的表达。以阴性对照慢病毒感染U-87 MG细胞作为对照组(4个细胞),以携带PWRN2序列的重组慢病毒(LV-PWRN2)感染U-87 MG细胞为试验组(4个细胞)。采用噻唑蓝法、细胞划痕实验分别检测上调PWRN2对U-87 MG细胞增殖和迁移能力的影响。采用qPCR检测上调PWRN2对Klotho基因mRNA表达的影响,通过蛋白质印记法检测Klotho蛋白和转化生长因子-β(TGF-β)信号通路蛋白的表达。结果PWRN2在胶质瘤组织中的表达水平低于癌旁组织[(1.25±0.52)比(6.28±0.66),P<0.01]。PWRN2在胶质瘤细胞株(U-87 MG、LN382、C6、SNB-19、U251)中的表达水平均低于正常脑胶质HEB细胞(均P<0.05),在U-87 MG细胞株的表达水平最低(P<0.01)。LV-PWRN2慢病毒感染细胞后,PWRN2表达水平明显增加[(10.28±0.84)比(1.02±0.12),P<0.01]。上调PWRN2可明显抑制细胞增殖能力(P<0.05)和迁移能力(P<0.01)。上调PWRN2可明显促进Klotho基因的表达[(4.33±0.53)比(1.01±0.09),P<0.01],抑制TGF-β信号通路蛋白的表达。结论PWRN2在胶质瘤组织及细胞株中呈低表达,上调PWRN2可抑制U-87 MG细胞的增殖和迁移,其作用机制可能是上调PWRN2促进Klotho基因的表达,抑制TGF-β信号通路的转导。Objective To observe the expression of long-chain noncoding RNA(lncRNA)PWRN2 in glioma tissue and its influence on glioma cell proliferation and migration,and to explore the possible mechanism of PWRN2.Methods Real-time fluorescence quantitative polymerase chain reaction(qPCR)was used to detect the expression levels of PWRN2 in 42 cases'glioma tissue and corresponding adjacent tissue,in 5 kinds of glioma cell lines,and in normal glial cells.The U-87MG cells infected by negative control lentiviruses were set as a control group,and the U-87 MG cells by recombinant lentiviruses(LV-PWRN2)carrying PWRN2 sequences as an experimental group.The effects of upregulated PWRN2 on the proliferation and migration of U-87 MG cells were detected by the thiazole blue method and cell scratch test.The effect of up-regulated PWRN2 on Klotho gene mRNA expression was detected by qPCR,and the expression levels of Klotho and TGFβsignaling pathway proteins were detected by Western blotting.Results The expression level of PWRN2 in the glioma tissue was lower than that in the corresponding adjacent tissue[(1.25±0.52)vs.(6.28±0.66),P<0.01].The expression levels of PWRN2 in the glioma cell lines(U-87 MG,LN382,C6,SNB-19,and U251)were lower than that in the normal brain glial HEB cells(all P<0.05),and the expression level in the U-87 MG cell lines was the lowest(P<0.01).After LV-PWRN2 lentiviruses infected the cells,the expression level of PWRN2 increased significantly[(10.28±0.84)vs.(1.02±0.12),P<0.01].The upregulation of PWRN2 could significantly inhibit the cell proliferation(P<0.05)and migration(P<0.01).The upregulation of PWRN2 could obviously promote the expression of Klotho gene[(4.33±0.53)vs.(1.01±0.09),P<0.01]and suppress the expression of TGFβsignaling pathway proteins.Conclusions PWRN2 is under-expressed in glioma tissue and cell lines.Upregulation of PWRN2 can inhibit the proliferation and migration of U-87 MG cells.The mechanism may be that upregulation of PWRN2 promotes the expression of Klotho gene and inhibits the trans

关 键 词：长链非编码RNA PWRN2 胶质瘤 肿瘤增殖 肿瘤转移 

分 类 号：R739.41[医药卫生—肿瘤]