非典型钙黏素1与食管鳞癌临床病理特征及预后的关系  

Association of FAT atypical cadherin 1 with clinicopathological parameters and prognosis in esophageal squamous cell carcinoma

作　　者：庄晓飞[1] 肖秀鹏 廉建红[1] 曹国珍[1] 杜小祥[1] 郭石平[1] Zhuang Xiaofei;Xiao Xiupeng;Lian Jianhong;Cao Guozhen;Du Xiaoxiang;Guo Shiping(Second Department of Thoracic Surgery,Shanxi Cancer Hospital,Taiyuan 030013,China)

机构地区：[1]山西省肿瘤医院胸外二科,太原030013

出　　处：《中华消化外科杂志》2021年第6期683-688,共6页Chinese Journal of Digestive Surgery

基　　金：山西省卫健委基金(2014049);山西省应用基础研究项目(201801D121309);山西省重点研发计划项目基金(201803D31171,201903D321168)。

摘　　要：目的探讨非典型钙黏素1(FAT1)与食管鳞癌临床病理特征及预后的关系。方法采用回顾性队列研究方法。收集2011年1月至2015年12月山西省肿瘤医院收治的124例食管鳞癌病人的临床病理资料;男85例,女39例;中位年龄为60岁,年龄范围为40~72岁。收集手术切除食管鳞癌组织及癌旁组织标本,制备组织芯片,行免疫组织化学染色检测。收集5对新鲜食管鳞癌组织及其相应癌旁组织进行实时荧光定量PCR(qRT-PCR)。观察指标:(1)食管鳞癌组织和癌旁组织中FAT1蛋白表达情况。(2)食管鳞癌组织和癌旁组织中FAT1 RNA表达情况。(3)食管鳞癌组织中FAT1蛋白表达及与病人临床病理因素的关系。(4)随访和生存情况。采用门诊和电话方式进行随访,了解病人生存情况。随访时间截至2019年2月13日。生存时间定义为手术日至病人肿瘤相关死亡或随访截止时间。正态分布的计量资料以x±s表示,组间比较采用t检验。偏态分布的计量资料以M(范围)表示。计数资料以绝对数或百分比表示,组间比较采用χ^(2)检验。等级资料比较采用非参数秩和检验。采用Kaplan-Meier法计算生存时间,采用Log-rank检验进行生存分析。结果(1)食管鳞癌组织和癌旁组织中FAT1蛋白表达情况:124例标本中,因组织脱落,最终获得食管鳞癌组织107例、癌旁组织93例;其中76例标本成功完成食管鳞癌及其癌旁组织配对。免疫组织化学染色检测结果显示:FAT1蛋白在食管鳞癌组织和癌旁组织中均有表达,阳性表达为棕黄色,FAT1定位在细胞膜,以FAT1的表达≥75为高表达,<75为低表达。76例食管鳞癌组织和癌旁组织中FAT1蛋白的相对表达量分别为68±42和77±37,两者比较,差异有统计学意义(t=2.380,P<0.05)。(2)食管鳞癌组织和癌旁组织中FAT1 RNA表达情况。qRT-PCR检测结果显示:5例食管鳞癌组织和癌旁组织标本中FAT1 RNA的相对表达量分别为1.6±0.4和2.5±0.3,两者�Objective To investigate the association of FAT atypical cadherin 1[FAT1]with clinicopathological parameters and prognosis in esophageal squamous cell carcinoma[ESCC].Methods The retrospective cohort study was conducted.The clinicopathological data of 124 patients with ESCC who were admitted to Shanxi Cancer Hospital from January 2011 to December 2015 were collected.There were 85 males and 39 females,aged from 40 to 72 years,with a median age of 60 years.The ESCC tissues surgically removed and adjacent tissues specimens were collected to prepare tissue microarray for immunohistochemical staining.The 5 cases of ESCC tissues and adjacent tissues were analyzed by real-time quantitative polymerase chain reaction[qRT-PCR].Observation indicators:[1]the expression of FAT1 protein in ESCC and adjacent tissues;[2]the expression of FAT1 RNA in ESCC and adjacent tissues;[3]the expression of FAT1 protein in ESCC tissues and its association with clinicopathological parameters;[4]follow-up and survival.Followup using outpatient examination and telephone interview was conducted to detect survival of patients up to February 13,2019.The survival time was from surgical date to tumor-related death or endpoint of follow-up.Measurement data with normal distribution were represented as Method and comparison between groups was analyzed using the t test Measurement data with skewed distribution were represented as M[range].Count data were described as absolute numbers or percentages,and comparison between groups was analyzed using the chi-square test.Comparison of ordinal data was analyzed using the non parameter rank sum test.The Kaplan-Meier method was used to calculate survival time,and Log-rank test was used for survival analysis.Results[1]The expression of FAT1 protein in ESCC and adjacent tissues:of 124 specimens,the 107 cases of ESCC tissues and 93 cases of adjacent tissues were finally obtained because of exfoliative tissues.There were 76 cases of ESCC tissues and corresponding adjacent tissues matched.Results of immunohistochem

关 键 词：食管肿瘤 非典型钙黏素1 免疫组织化学染色 组织芯片 预后 

分 类 号：R735.1[医药卫生—肿瘤]