基于微流控技术的液滴微颗粒分选  

Droplet Micro-Particle Separation Based on Microfluidic Technology

作　　者：钟明浩 郭钟宁[1] 刘宇迅 Zhong Minghao;Guo Zhongning;Liu Yuxun(School of Electromechanical Engineering,Guangdong University of Technology,Guangzhou 510000,China)

机构地区：[1]广东工业大学机电工程学院,广州510000

出　　处：《微纳电子技术》2021年第4期332-336,共5页Micronanoelectronic Technology

摘　　要：微流控技术在医疗分析中具有重要的应用意义。在对样品生物分子分析前通常需要进行分选,达到纯化的目的。使用不同种类的微颗粒代替生物分子(如细胞、蛋白质和DNA等),可模拟其在海藻酸钠溶液环境中的作用。通过软光刻制备的微流控芯片集成了微颗粒聚焦、液滴包裹微颗粒、液滴减速及液滴颗粒分选4个功能。通过鞘液流使微颗粒聚焦成一行,可有效提高海藻酸钠液滴包裹单颗颗粒的概率,且在分选前通过分流使液滴减速更加易于将目标颗粒精准分选。其中海藻酸钠液滴包裹有单颗颗粒的概率为21%,空液滴的概率为79%。通过电分选技术成功将包裹有荧光微颗粒的液滴从样品中分选出来,分选成功率达到了87%,可为医疗分析提供技术支持,降低医疗检测成本。Microfluidic technology has important application significance in medical analysis.Before the analysis of sample biomolecules,separation is usually required to achieve the purpose of purification.Their effects in sodium alginate solution was simulated by using different kinds of micro-particles instead of biomolecules(such as cells,proteins,DNA,etc.).The microfluidic chip fabricated by soft lithography integrated four functions:micro-particles focusing,encapsulation of micro-particles into droplets,droplet deceleration and droplet particle separation.The micro-particles were focused into a line by sheath flow to effectively improve the probability of sodium alginate droplets encapsulating single particle.The droplets were slowed down using bypass before separation to make it easier to accurately separate the target particles.The probability of sodium alginate droplets with single particle is 21%,and the probability of empty droplets is79%.The droplets with fluorescent micro-particles are successfully separated from the samples by the electrical separation technology,and the separation success rate reaches 87%,which can provide technical support for medical analysis and reduce the cost of medical detection.

关 键 词：海藻酸钠 微流控技术 微颗粒 液滴 分选 软光刻 

分 类 号：O35[理学—流体力学] TH703[理学—力学]