大鼠骨髓基质细胞分化为施万细胞样细胞过程中的基因表达变化  

Analysis of gene expression profile during the differentiation of bone marrow stromal cells into Schwann-like cells

作　　者：陈娇 张丹丹 李军平[1] 牛建国[1] 何仲义[1] 贾桦[1] Chen Jiao;Zhang Dandan;Li Junping;Niu Jianguo;He Zhongyi;Jia Hua(Department of Human Anatomy and Histoembryology,School of basic Medicine,Ningxia Medical University,Yinchuan 750004;Department of Pathophysiology,Chengde Medical College,Chengde 067000,China)

机构地区：[1]宁夏医科大学基础医学院人体解剖与组织胚胎学系,银川750004 [2]承德医学院病理生理学教研室,承德067000

出　　处：《神经解剖学杂志》2021年第3期315-322,共8页Chinese Journal of Neuroanatomy

基　　金：宁夏自然科学基金(2021AAC03150)。

摘　　要：目的:利用转录组测序技术(RNA-seq)检测骨髓基质细胞(BMSCs)分化为施万细胞(SCs)样细胞过程中基因表达谱的变化,筛选与分化相关的差异表达基因,并分析与分化相关的信号通路。方法:体外培养大鼠BMSCs,用三步诱导法对其进行诱导,在复合生长因子诱导第12 h和7 d收集样本。经Illumina测序平台对BMSCs、诱导培养基(DM)诱导12 h(DM-12 h)和7 d(DM-7 d)的细胞进行生物信息分析,采用Cluster Profiler软件对差异基因集进行GO功能和KEGG代谢通路富集分析。结果:与BMSCs组相比,DM-12 h组有578个基因表达上调,632个基因表达下调;与DM-12 h组相比,DM-7 d组有1165个基因表达上调,872个基因表达下调;与BMSCs组相比,DM-7 d组有629个基因表达上调,529个基因表达下调。经Venn分析,共筛选出140个在各阶段共同表达的差异基因,其中与分化相关的基因主要富集在细胞周期、DNA复制和p53信号通路;并确定Ccne2、Mcm3和Rrm2可作为下一步研究的候选基因。结论:本研究首次基于转录组测序构建BMSCs诱导分化为SCs样细胞的基因表达谱,揭示分化过程中的差异基因,并筛选出Ccne2、Mcm3和Rrm2作为研究目的基因,为阐明BMSCs分化为SCs样细胞的机制提供依据。Objective:RNA-sequence(RNA-seq)was utilized to detect the changes of genes expression profile during the differentiation of bone marrow stromal cells(BMSCs)into Schwann-like cells,as well the differentially expressed genes related to differentiation were screened and the signal pathways were analyzed.Methods:BMSCs derived from rats were cultured in vitro,and then were induced by three-steps induction method.The samples were collected at 12 h and 7 d after induction by the compound growth factors.Analyze the bioinformatics of BMSCs and the cells induced by differentiation media(DM)for 12 h(DM-12 h)and 7 d(DM-7 d)by Illumina sequencing platform.The gene ontology(GO)function and kyoto encyclopedia of genes and genomes(KEGG)metabolic pathway enrichment analysis of the differential gene sets were conducted by Cluster Profile software.Results:Compared with BMSCs group,632 genes were up-regulated and 578 genes were down-regulated in DM-12 h group.1165 genes were up-regulated and872 genes were down-regulated in DM-7 d group compared with DM-12 h group.529 genes were up-regulated and 629 genes were down-regulated in DM-7 d group by contrast to BMSCs group.Venn analysis showed that 140 differentially expressed genes at various stages were screened,and the gene enrichment mainly existed in cell cycle,DNA replication and p53 signal pathway.Besides,Ccne2,Mcm3,and Rrm2 could be used as candidate genes for the further research.Conclusion:The genes expression profile of BMSCs induced into Schwann-like cells was constructed based on RNA-seq for the first time,and the differential genes in the process of differentiation were revealed.Ccne2,Mcm3 and Rrm2 were screened as the target genes,which provided a basis for elucidating the mechanism of BMSCs differentiation into Schwann-like cells.

关 键 词：骨髓基质细胞 施万细胞样细胞 分化 转录组测序 大鼠 

分 类 号：R-33[医药卫生]