星康吉鳗发育过程中个体形态学和卵母细胞特征变化及组织营养成分分析  

Changes of individual morphology and oocyte characteristics and analysis of tissue nutrient content during the ovary development of conger eel(Conger myriaster)

作　　者：吴盟 鲁强 刘如聪 江兴龙[2] 宋宗诚 李慷[1,4] 刘利平[1,4] WU Meng;LU Qiang;LIU Rucong;JIANG Xinglong;SONG Zongcheng;LI Kang;LIU Liping(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Engineering Research Center of the Modern Technology for Eel Industry,Ministry of Education,Xiamen 361021,Fujian,China;Weihai Shenghang Aquatic Technology Limited Company,Weihai 264299,Shandong,China;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306 [2]鳗鲡现代产业技术教育部工程研究中心,福建厦门361021 [3]威海圣航水产科技有限公司,山东威海264299 [4]上海海洋大学上海水产养殖工程技术研究中心,上海201306

出　　处：《上海海洋大学学报》2021年第3期442-453,共12页Journal of Shanghai Ocean University

基　　金：中国-东盟海上合作基金项目(DF);鳗鲡现代产业技术教育部工程研究中心开放基金(RE201901);上海市科技兴农项目(2020-02-08-00-10-F01471)。

摘　　要：对星康吉鳗(Conger myriaster)卵巢发育过程中的个体形态学和卵母细胞特征变化,以及卵巢、肝脏、肌肉中营养成分的变化情况进行研究。结果表明:星康吉鳗卵巢发育分为5个时期,即卵母细胞单层滤泡期、卵母细胞出现脂肪泡期、卵母细胞卵黄充满期(早期、中期、末期)、卵母细胞核极化期和卵母细胞成熟期。卵母细胞直径、细胞核直径、脂肪泡直径和卵黄球直径随着卵母细胞的发育逐渐增长。卵母细胞中脂肪泡的初始发生位点在细胞核周围,并且发生时间上脂肪泡先于卵黄球,随着卵母细胞发育,脂肪泡呈环状分布并逐渐向细胞膜方向延伸;卵母细胞中卵黄球的初始发生位点在细胞膜周边的细胞质层,随着卵母细胞发育,卵黄球呈环状分布并向细胞核方向延伸。发育过程中星康吉鳗性体指数、肝体指数、眼径指数、肛长指数之间均呈正相关性;消化道指数与性体指数、肝体指数、眼径指数、肛长指数分别呈负相关性。营养成分变化表明,星康吉鳗主要储存脂肪的位置在肌肉,脂肪的转运在星康吉鳗的卵巢发育中起到了重要作用。研究结果可为星康吉鳗全人工繁殖提供参考。The changes of individual morphology and oocyte characteristics of conger eel during the development of ovary were studied;meanwhile,the nutritional components of ovary,liver and muscle were investigated.The results showed that the ovary development of conger eel could be divided into 5 stages:the single-layer follicular cells stage,the oil drop stage,the yolk filled stage(early,middle and late),the nucleus polarized stage,and the mature oocyte stage.The diameter of the oocyte,nucleus,oil drop,and the yolk globule gradually increased with the development of the oocyte.The initial location of oil drops was around the nucleus,and preceded the yolk sphere.The oil drops were distributed in a ring shape and gradually extended toward the cell membrane through the oocyte development.The initial occurrence site of yolk sphere was in the cytoplasmic layer around the cell membrane,which was distributed in a ring shape as the oocyte developed and extended toward the nucleus.The gonadosomatic index,hepatosomatic index,ocular index,and anal length index all showed positive correlations during development.The digestive gut index was negatively correlated with gonadosomatic index,hepatosomatic index,ocular index,and anal length index.The changes in nutritional composition indicated that the fat storage location is in muscle,and the fat transportation plays a critical role during the ovary development.The results of this study give an essential reference for the artificial reproduction of conger eel.

关 键 词：星康吉鳗 性腺发育 卵母细胞 个体形态学 营养成分 

分 类 号：S917[农业科学—水产科学]