济南市2012~2017年手足口病4种优势肠道病毒阳性及VP1基因型分布  

Positive distribution of four dominant enteroviruses associated w ith hand,foot and mouth disease and VP1 genotypes in Jinan,China,2012-2017

作　　者：关恒云[1] 王春荣[1] 刘岚铮[1] 赵怀龙[1] 白爱英[1] 姜慧钰 董华 李娜 单晓英[1] 成洪旗[1] GUAN Hengyun;WANG Chunrong;LIU Lanzheng;ZHAO Huailong;BAI Aiying;JIANG Huiyu;DONG Hua;LI Na;SHAN Xiaoying;CHENG Hongqi(Bacterial Disease Inspection Laboratory,Jinan Center for Disease Control and Prevention,Jinan 250021,Shandong,China)

机构地区：[1]济南市疾病预防控制中心细菌性疾病检验所,山东济南250021

出　　处：《山东大学学报（医学版）》2021年第5期60-67,共8页Journal of Shandong University:Health Sciences

基　　金：山东省医药卫生科技发展计划(2016WS0116)。

摘　　要：目的阐明济南市手足口病优势肠道病毒感染类型及VP1基因型分布。方法采用实时荧光定量逆转录-聚合酶链反应(RT-PCR)对2012~2017年济南市4 929份手足口病病例标本进行肠道病毒71型(EV-A71)、柯萨奇病毒A组16型(CV-A16)、柯萨奇病毒A组6型(CV-A6)和柯萨奇病毒A组10型(CV-A10)进行鉴定。按照不同年份回顾性收集手足口病病毒分离株65株,包括17株EV-A71、16株CV-A16、20株CV-A6和12株CV-A10。采用RT-PCR方法扩增肠道病毒VP1特异性基因并进行核苷酸序列测定;获得的目的基因序列进行核苷酸同源性分析并构建系统进化树。结果济南市手足口病优势肠道病毒类型为CV-A16(28.3%)、EV-A71(27.3%)、CV-A6(17.0%)、CV-A10(3.0%)。4种优势肠道病毒VP1基因目的片段长度分别为891 bp、1 015 bp、1 110 bp和993 bp。济南市EV-A71病毒株间核苷酸序列同源性为93.0%~98.1%,CV-A16为87.4%~100.0%,CV-A6为87.6%~99.7%,CV-A10为95.8%~99.4%。系统进化树分析显示,济南市EV-A71均为C4a亚型;CV-A16存在B1b型和B1a型两种基因亚型;CV-A6在系统进化树的C簇、E簇和F簇均有分布;CV-A10全部位于进化树E簇。结论 CV-A16、EV-A71、CV-A6和CV-A10为济南市2012~2017年手足口病的优势肠道病毒;长期动态监测肠道病毒VP1基因型分布,对于提高手足口病预警敏感性具有重要的公共卫生意义。Objective To elucidate the dominant enteroviruses associated with hand,foot and mouth disease(HFMD) and the distributions of VP1 genotypes in Jinan City. Methods Real-time quantitative reverse transcription-polymerase chain reaction(RT-PCR) was used to detect enterovirus A71(EV-A71),coxsackievirus A16(CV-A16),coxsackievirus A6(CV-A6) and coxsackievirus A10(CV-A10) in 4 929 HFMD specimens from 2012 to 2017 in Jinan. According to different years,65 virus isolates of HFMD were retrospectively collected,including 17 strains of EV-A71,16 of CV-A16,20 of CV-A6 and 12 of CV-A10. The VP1 gene of enteroviruses was amplified by RT-PCR and the nucleotide sequencing was performed. Nucleotide homology analyses were performed and phylogenetic trees were constructed on the VP1 gene. Results The dominant enteroviruses of HFMD were CV-A16(28.3%),EV-A71(27.3%),CV-A6(17.0%) and CV-A10(3.0%) in Jinan. The lengths of VP1 gene on CV-A16,EV-A71,CV-A6 and CV-A10 amplified by RT-PCR were 891 bp,1 015 bp,1 110 bp and 993 bp,respectively. The nucleotide homology was 93.0%-98.1% for EV-A71 strains,87.4%-100.0% for CV-A16 strains,87.6%-99.7% for CV-A6 strains,and 95.8%-99.4% for CV-A10 strains. The phylogenetic trees showed that EV-A71 strains belonged to C4 a genotype in Jinan,CV-A16 strains had two genetic subtypes(B1 b and B1 a);CV-A6 strains located in clusters C,E and F,and CV-A10 strains located in cluster E. Conclusion The dominant enteroviruses of HFMD are CV-A16,EV-A71,CV-A6 and CV-A10 from 2012 to 2017 in Jinan. The long-term dynamic monitoring of the distribution of VP1 genotypes is of great public health significance for improving the early warning sensitivity for HFMD.

关 键 词：手足口病 肠道病毒 VP1基因 系统进化分析 

分 类 号：R115[医药卫生—公共卫生与预防医学]