不同制样方法下四种豆科植物花粉的扫描电镜观察  

Pollen observation of four Fabeceae species under scanning electron microscopy using different drying method

作　　者：赵颖 ZHAO Ying(Center for Integrative Conservation,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Mengla Yunnan 666303;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院西双版纳热带植物园综合保护中心,勐腊云南666303 [2]中国科学院大学,北京100049

出　　处：《电子显微学报》2021年第3期307-310,共4页Journal of Chinese Electron Microscopy Society

基　　金：中国科学院重大科技基础设施开放研究项目(No.2017-LSFGBOWS-02)。

摘　　要：本研究采用自然干燥法和DMP(2,2-二甲氧基丙烷)制样法两种方法,分别对渐尖羊蹄甲、铁刀木、洋金凤和云南火索藤四种豆科植物的花粉材料进行干燥处理,然后在扫描电镜下观察并分析花粉形态特征差异。结果表明,DMP制样法比自然干燥法处理的花粉更少变形,且花粉表面和周围具有更少的杂质,整体成像效果更好,更能准确展示花粉的形态特征。Pollen morphological characteristics of four Fabeceae species,including Bauhinia acuminata,Senna siamea,Caesalpinia pulcherrim and Phanera yunnanensis were obtained by scanning electron microscopy(SEM)using natural drying and 2,2-Dimethoxypropane(DMP)treatment method,respectively.The results showed that compared with natural drying,the pollen material prepared via DMP treatment was less out of shape and provided more details of the exine.Here,we suggest that the DMP treatment method could be a useful and effective method for the living pollen material preparation of SEM.

关 键 词：花粉 扫描电镜 自然干燥法 DMP(2 2-二甲氧基丙烷)制样法 

分 类 号：Q944[生物学—植物学] Q336