非编码小RNA200家族及U78在老年口腔鳞癌病人组织中的表达及功能分析  

Expression and functional analysis of non-coding microRNA-200 family and U78 in elderly patients with oral squamous cell carcinoma

作　　者：房旭 黄郁晶[2] 阮强[2] 刘维贤[1] FANG Xu;HUANG Yu-jing;RUAN Qiang;LIU Wei-xian(Department of Oral and Maxillofacial Surgery,Shengjing Hospital of China Medical University,Shenyang 110004,China;Virus Laboratory,Shengjing Hospital of China Medical University,Shenyang 110004,China)

机构地区：[1]中国医科大学附属盛京医院口腔颌面外科,辽宁省沈阳市110004 [2]中国医科大学附属盛京医院病毒研究室,辽宁省沈阳市110004

出　　处：《实用老年医学》2021年第6期555-559,共5页Practical Geriatrics

基　　金：国家自然科学基金资助项目(81371788)。

摘　　要：目的筛选并鉴定年龄≥60岁老年口腔鳞癌(OSCC)病人的癌组织及正常组织中的非编码小RNA(microRNA)的表达,分析其在OSCC发生中的临床意义。方法收集44例老年OSCC标本,23例正常口腔组织标本进行芯片检测,筛选与OSCC密切相关的micro RNA。采用Taqman定量PCR方法检测OSCC组织与正常组织中microRNA-200家族(miR-200a、miR-200b、miR-200c、miR-141和miR-429)及U78的转录水平,对检测结果进行统计学分析,并采用生物信息学工具对其生物学功能进行预测分析。结果芯片及Taqman定量PCR结果证实,与正常口腔组织相比,OSCC组织中microRNA-200家族各microRNAs及U78的表达水平均有显著升高。生物信息学分析结果提示,microRNA-200家族microRNAs可靶向调控β-链蛋白和转化生长因子β2等与肿瘤发生发展密切相关的基因。多元线性回归分析表明,U78的表达量与肿瘤组织病理分级具有明显相关性(P=0.023),与临床分期及有无临床转移等临床指征无相关性。结论microRNA-200家族及U78作为生物诊断指标可用于辅助诊断OSCC。Objective To screen and identify the expression of non-coding microRNAs in oral squamous cell carcinoma(OSCC)and normal tissues in the elderly patients,and to analyze the clinical significance in the occurrence of OSCC.Methods A total of 44 OSCC specimens and 23 oral tissue specimens were collected,and the expression of non-coding microRNAs closely related to OSCC were screened.The transcription levels of microRNAs 200 family(miR-200a,miR-200b,miR-200c,miR-141 and miR-429)and U78 in OSCC tissues and normal tissues were detected by TaqMan quantitative PCR method.Then,the results were analyzed by statistical methods.Bioinformatics tools were used to predict the biological function.Results Microarray and TaqMan quantitative PCR results confirmed that the expression levels of microRNA-200 family and U78 in OSCC tissues were significantly higher than those in normal oral tissues.Bioinformatics analysis indicated that microRNA-200 family could target genes closely related to tumor development,such asβ-catenin and transforming growth factorβ2.Multiple linear regression analysis showed that the expression of U78 was significantly correlated with pathological grade(P=0.023),but not with clinical stage and metastasis.Conclusions MicroRNA-200 family and U78 may be involved in the occurrence of OSCC and may be potential biomarkers for the clinical diagnosis of OSCC.

关 键 词：口腔鳞状细胞癌 microRNA-200家族 U78 

分 类 号：R739.85[医药卫生—肿瘤]