干姜化学成分的分离及抗肿瘤活性成分筛选  

Isolation of Chemical Constituents and Screening of Antitumor Active Components of Rhizoma Zingiberis

作　　者：陈靖[1] 柴玲 谭敏[1] CHEN Jing;CHAI Ling;TAN Min(Department of Pharmacy,Changde Vocational Technical College,Hunan Changde 415000;Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards,Guangxi Institute of Chinese Medicine&Pharmaceutical Sciences,Guangxi Nanning 530022,China)

机构地区：[1]常德职业技术学院药学系,湖南常德415000 [2]广西中医药研究所广西中药质量标准研究重点实验室,广西南宁530022

出　　处：《广州化工》2021年第12期111-113,134,共4页GuangZhou Chemical Industry

基　　金：干姜附子汤中抗肿瘤成分的研究(ZY1623,2019ZD31)。

摘　　要：采用分析型HPLC累积进样富集馏分的方法分离纯化干姜姜油树脂的主要化学成分,将得到的化合物用TOF/MS质谱进行鉴定,从姜油树脂中分离得到5个化合物,分别为6-gingerol;8-gingerol;diacetoxy-6-gingerdiol;6-shogaol和10-gingerol。利用MTT法对单体化合物及干姜提取物进行体外抗肿瘤活性筛选。MTT试验结果表明,6-gingerol,6-shogaol及干姜提取物对肝癌细胞均有细胞毒性,其中6-shogaol对肝癌细胞有细胞毒性且呈浓度依赖性,对SMMC-7721细胞毒性最为明显,20μM 6-shogaol作用24 h约抑制50%SMMC-7721细胞增殖。The main chemical components of ginger oleoresin were separated and purified by the method of analytical HPLC,and the compounds were identified by TOF/MS mass spectrometry.Five compounds were isolated from ginger oleoresin and identified as 6-gingerol,8-gingerol,diacetoxy-6-gingerol,6-shogaol and 10-gingerol.MTT assay was used to screen the antitumor activity of the monomers and the extract of ginger in vitro.The results showed that 6-ginger,6-shogaol and dried ginger extracts had cytotoxicity to hepatoma cells.Among them,6-shogaol had cytotoxicity on hepatoma cells in a concentration dependent manner,and the most obvious toxicity was SMMC-7721 cells.20μm 6-shogaol inhibited the proliferation of SMMC-7721 cells by 50%for 24 h.

关 键 词：干姜 姜油树脂 化学成分 抗肿瘤活性 

分 类 号：R284.2[医药卫生—中药学]