饲粮添加干酪乳杆菌代田株对断奶仔猪生长性能、抗氧化功能和结肠生物胺的影响  

Effects of Lactobacillus casei Strain Shirota on Growth Performance,Antioxidant Function and Bioamines in Colon of Weaned Piglets

作　　者：饶泽斌 曾艳 刘宇波 胡萍[1] 胡红[1] 唐志如[1] RAO Zebin;ZENG Yan;LIU Yubo;HU Ping;HU Hong;TANG Zhiru(Laboratory for Animal Nutrition and Bio-Feed,College of Animal Science and Technology,Southwest University,Chongqing 400715,China;Hunan Institute of Microbiology,Changsha 410000,China)

机构地区：[1]西南大学动物科学技术学院,动物营养与生物饲料实验室,重庆400715 [2]湖南省微生物研究院,长沙410000

出　　处：《动物营养学报》2021年第6期3165-3175,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31772610);国家重点研发计划项目(2018YFD0501000);重庆市留学人才创新计划重点项目(cx2017024)。

摘　　要：本试验旨在研究干酪乳杆菌代田株(Lactobacillus casei strain Shirota)对断奶仔猪生长性能、抗氧化功能和结肠生物胺的影响。选择30只健康的28日龄“杜×长×大”断奶仔猪,随机分为3个组,每组10个重复,每个重复1头猪。对照组饲喂基础饲粮,抗生素组饲喂基础饲粮+100 mg/kg硫酸黏杆菌素,干酪乳杆菌组饲喂基础饲粮+每天口服用琼脂块包埋的5×109 CFU干酪乳杆菌代田株。预试期4 d,正试期28 d。结果表明:1)与对照组相比,干酪乳杆菌组断奶仔猪末重、平均日增重和平均日采食量显著提高(P<0.05),血浆总抗氧化能力及总超氧化物歧化酶、一氧化氮合酶(NOS)、谷胱甘肽过氧化物酶活性极显著提高(P<0.01),肝脏NOS活性极显著提高(P<0.01),空肠杯状细胞数量显著增加(P<0.05),血浆谷草转氨酶(GOT)和谷丙转氨酶活性极显著降低(P<0.01),肝脏GOT活性显著提高(P<0.05),结肠内容物色胺、甲胺、组胺、尸胺和腐胺含量极显著降低(P<0.01)。2)与抗生素组相比,干酪乳杆菌组断奶仔猪血浆GOT活性显著提高(P<0.05),血浆和肝脏过氧化氢酶和NOS活性极显著提高(P<0.01),空肠杯状细胞数量显著增加(P<0.05),结肠内容物组胺含量极显著降低(P<0.01)。综上所述,饲粮添加干酪乳杆菌代田株能够提高断奶仔猪生长性能和抗氧化功能,降低结肠内容物生物胺含量,具有替代抗生素的功效。This experiment was conducted to investigate the effects of Lactobacillus casei strain Shirota on growth performance,antioxidant function and bioamines in colon of weaned piglets.Thirty healthy 28-day-old Duroc×Landrace×Yorkshire weaned piglets were randomly divided into 3 groups with 10 replicates per group and 1 pig per replicate.Piglets in the control group were fed a basal diet,those in the antibiotic group were fed the basal diet+100 mg/kg colistin sulfate,and those in the Lactobacillus casei group were fed the basal diet+5×109 CFU Lactobacillus casei strain Shirota embedded with agar block for oral administration every day.The pre-experiment lasted for 4 days,and the experimental period lasted for 28 days.The results showed as follows:1)compared with the control group,the final weight,average daily gain and average daily feed intake of weaned piglets in the Lactobacillus casei group were significantly increased(P<0.05),the total antioxidant capacity and the activity of total superoxide dismutase,nitric oxide synthase(NOS)and glutathione peroxidase in plasma were extremely significantly increased(P<0.01),the NOS activity in liver was extremely significantly increased(P<0.01),the number of goblet cells in jejunum was significantly increased(P<0.05),the activity of glutamic oxalacetic transaminase(GOT)and glutamic-pyruvic transaminase in plasma was extremely significantly decreased(P<0.01),the GOT activity in liver was significantly increased(P<0.05),and the contents of tryptamine,methylamine,histamine,cadaverine and putrescine in colon were extremely significantly decreased(P<0.01).2)Compared with the antibiotic group,the GOT activity in plasma of weaned piglets in the Lactobacillus casei group was significantly increased(P<0.05),the activity of catalase and NOS in both plasma and liver was extremely significantly increased(P<0.01),the number of goblet cells in jejunum was significantly increased(P<0.05),and the histamine content in colon was extremely significantly decreased(P<0.01).In conclusion,dietary

关 键 词：干酪乳杆菌代田株 断奶仔猪 生长性能 抗氧化功能 肠道发育 

分 类 号：S816.7[农业科学—饲料科学]