北虫草不同极性部位体外抗肿瘤活性研究  

Study on In Vitro Anti-tumor Activity of Different Polar Parts of Cordyceps Militaris

作　　者：李万芳[1] 宋健[2] 李洁 LI Wan-fang;SONG Jian;LI Jie(Shandong Xiehe College,Jinan 250109,China;Shandong University of Traditional Chinese Medicine,Jinan 250355,China;College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东协和学院,山东济南250109 [2]山东中医药大学,山东济南250355 [3]山东中医药大学中医学院,山东济南250355

出　　处：《食品与药品》2021年第3期211-214,共4页Food and Drug

基　　金：山东省中医药科技发展计划项目(编号:2015-310,2015-032,2017-278)。

摘　　要：目的研究山东北虫草不同极性部位体外抗肿瘤活性。方法采用系统溶剂法,分别用石油醚、乙酸乙酯、正丁醇和蒸馏水依次提取北虫草不同极性部位。以乳腺癌MCF-7细胞、食管癌ECA-109细胞和肺癌A549细胞为研究对象,用MTT法检测北虫草不同极性部位对MCF-7细胞、ECA-109细胞和A549细胞的抑制作用。结果石油醚部位在100μg/ml浓度时,对MCF-7细胞、ECA-109细胞和A549细胞的抑制率较强,乙酸乙酯部位在50,100μg/ml浓度对ECA-109细胞的抑制率较强。结论北虫草石油醚部位和乙酸乙酯部位体外抗肿瘤活性较强,值得进一步研究其化学成分。Objective To study the in vitro anti-tumor activity of different polar parts of Cordyceps militaris in Shandong.Methods Using the systematic solvent method,the different polar parts were extracted with petroleum ether,ethyl acetate,n-butanol and distilled water.Taking breast cancer MCF-7 cells,esophageal cancer ECA-109 cells and lung cancer A549 cells as research objects,the inhibitory effects of different polar parts of Cordyceps militaris were detected by MTT method.Results When the concentration of petroleum ether extract was 100μg/ml,the inhibition rates for MCF-7 cells,ECA-109 cells and A549 cells were high.When the concentration of ethyl acetate extract was 50μg/ml or 100μg/ml,the inhibition rate for ECA-109 cells was high.Conclusion The in vitro anti-tumor activity of polar parts of Cordyceps militaris extracted by petroleum ether and ethyl acetate is strong and it is worth further study of its chemical composition.

关 键 词：北虫草 系统溶剂法 抗肿瘤活性 

分 类 号：R284.2[医药卫生—中药学]