椰心叶甲啮小蜂转录组分析及基因功能注释  

Transcriptome Analysis and Gene Function Annotation of Tetrastichus brontispae

作　　者：刘华伟 李朝绪[2,4,5] 李芬 吕朝军[2,4,5] 吴少英[3] 覃伟权 LIU Huawei;LI Chaoxu;LI Fen;LU Chaojun;WU Shaoying;QIN Weiquan(College of Tropical Crops,Hainan University,Haikou 570228,China;Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571399,China;College of Plant Protection,Hainan University,Haikou 570228,China;Hainan Innovation Center of Academician Team,Haikou 571339,China;Hainan Key Laboratory of Tropical Oil Crops Biology,Haikou 571399,China)

机构地区：[1]海南大学热带作物学院,海口570228 [2]中国热带农业科学院椰子研究所,海口571399 [3]海南大学植物保护学院,海口570228 [4]海南省院士团队创新中心,海口571339 [5]海南省热带油料作物生物学重点实验室,海口571399

出　　处：《中国生物防治学报》2021年第3期412-419,共8页Chinese Journal of Biological Control

基　　金：海南省重大项目(ZDKJ201817);热带木本油料产业技术创新团队项目(1630152017010);海南大学基金(KYQD(ZR)1963,KYQD(ZR)1951);农业农村部海南省入侵生物监测项目(1630042020020)。

摘　　要：椰心叶甲啮小蜂Tetrastichus brontispae Ferrière可以有效地防治椰心叶甲Brontispa longissima(Gestro)危害,但是由于缺乏相关的遗传信息,其分子生物学的相关研究仍然较少。本研究通过Illumina测序平台,对椰心叶甲啮小蜂进行转录组测序。经DeNovo拼接后,共获得29535条Unigene,进一步将Unigene与NR、GO、eggNOG和KEGG四个数据库进行比对,结果表明,共有13796条Unigene被注释。在NR数据库中注释到13401条Unigene,与丽蝇蛹集金小蜂Nasonia vitripennis相似的基因序列最多;根据GO数据库,将3834条Unigene归类为三大类67个功能组;eggNOG数据库将12707条Unigene分25个功能分类;KEGG数据库将5999个Unigene分为五大类。对椰心叶甲啮小蜂进行转录组测序和基因功能注释,可以为今后研究该蜂的遗传多样性、基因功能挖掘等提供数据支撑。Tetrastichus Brontispae Ferrière,an effective parasitoid of Brontispa longissima(Gestro),is less studied in molecular biology due to the lack of relevant genetic information.In this study,transcriptome sequencing was performed on T.brontispae.After splicing with De Novo,29535 Unigenes were obtained,of which 13796 Unigenes were annotated with further comparison of Unigene with four public databases of NR,GO,eggNOG and KEGG..From the NR database,13401 Unigenes were annotated in T.brontispae,showing the most gene sequences similar with Nasonia Vitripennis.According to GO database,3834 Unigenes were classified into 67 functional groups in three categories;based on EggNOG database,12707 Unigenes were categorized into 25 functional groups;and according to KEGG database,5999 Unigenes were classified into five categories.The transcriptome sequencing and gene functional annotation of this study can provide data support for future research on genetic diversity and gene function mining.

关 键 词：椰心叶甲啮小蜂 转录组分析 功能注释 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]