上皮膜蛋白1在鼻咽癌组织中的表达特征  

Expressive features of epithelial membrane protein 1 in nasopharyngeal carcinoma

作　　者：张虹[1] 张春[2] 肖志勇[1] ZHANG Hong;ZHANG Chun;XIAO Zhiyong(Department of Otorhinolaryngology Head and Neck Surgery,the Affiliated Fuzhou Municipal Se-cond Hospital,Xiamen University,Fuzhou,Fujian 350007,China)

机构地区：[1]厦门大学附属福州市第二医院耳鼻咽喉头颈外科,福州350007 [2]福建医科大学附属福建省肿瘤医院头颈放疗科

出　　处：《福建医药杂志》2021年第3期14-18,共5页Fujian Medical Journal

摘　　要：目的探讨上皮膜蛋白1(EMP1)在鼻咽癌组织与正常鼻黏膜组织中的表达差异及其与疾病发展的关系.方法收集40例鼻咽癌患者的癌组织(实验组)与正常鼻黏膜组织(对照组)各40份标本,RT-qPCR、免疫荧光染色和免疫印记技术检测EMP1在两种组织中的表达情况;40例鼻咽癌标本免疫印迹测定EMP1灰度值与其对应的肿瘤分化程度、临床分期进行二元线性回归分析.结果与正常鼻黏膜上皮对比,鼻咽癌上皮细胞的EMP1表达明显降低(P<0.05),肿瘤细胞EMP1的表达与肿瘤分化程度呈正相关(P<0.05),与临床分期不存在线性关系(P>0.05).结论EMP1在鼻咽癌细胞和正常鼻黏膜细胞表达有差异,其值可能预示肿瘤分化程度.Objective To investigate the expressive differences of epithelial membrane protein 1(EMP1)between naso-pharyngeal carcinoma tissues and normal nasal mucosa tissues and its relationship with disease development.Methods The ex-pression of EMP1 in 40 nasopharyngeal carcinoma tissues(experimental group)and normal nasal mucosa tissues(control group)were detected by RT-qPCR,immunofluorescence staining and immunoblotting.The gray value of EMP1 was determined by Western blot in 40 nasopharyngeal carcinoma specimens,and the binary linear regression analysis was performed with tumor dif-ferentiation degree and clinical stage.Results Compared with normal nasal mucosa epithelium cells,the expression of EMP1 in nasopharyngeal carcinoma epithelial cells was significantly decreased(P<0.05).The expression of EMP1 in tumor cells was positively correlated with the degree of tumor differentiation(P<0.05),but there was no significant linear relationship between EMP1 expression and clinical stage(P>0.05).Conclusion The expression of EMP1 in nasopharyngeal carcinoma cells is di-fferent from that in normal nasal mucosa cells,which may predict the degree of tumor differentiations.

关 键 词：上皮膜蛋白1(EMP1) 鼻咽癌 紧密连接 

分 类 号：R739.63[医药卫生—肿瘤]