中耳黏膜的扫描电镜样品制备方法的改进  

The improvement of the preparation method of SEM samples of middle ear mucosa

作　　者：姬曼 赵君朋 Ji Man;Zhao Junpeng(Core Facility Center,CapitaI Medical Uni versity.Beijing 100069,China)

机构地区：[1]首都医科大学中心实验室,100069

出　　处：《首都食品与医药》2021年第12期109-110,共2页Capital Food Medicine

摘　　要：本文通过非酸脱钙剂乙二胺四乙酸二钠(EDTA)处理改进大鼠中耳黏膜扫描电镜样品制备方法 ,观察中耳黏膜细胞纤毛及其表面结构.结果 显示,经EDTA脱钙制备处理后中耳黏膜表面超微结构保存良好,鼓室及咽鼓管中纤毛细胞结构特征显示清晰,且样品结构的完整性保存良好.此方法 制备简便,效果良好,为与骨性结构关系密切软组织扫描电镜样品制备提供了一种科学有效的制备方法 .In this paper,it improved the preparation method of scanning electron microscope(SEM)samples of middle ear mucosa by the treatment of EDTA.and then observed the cell eilia and surface strueture of middle ear epithelium mucosa.The resuls showed that it was clear of surface ultrastrueture characteristics of ciliated rells in the middle ear epithelium mucosa.meanwhile it was well preserved of the integrity of sample structure such as tympanum and eustachian tube.This method is simple and effective,and it provides a scientifie and ffeetive method for the preparation of SEM samples of soft tissue closely rlaterd to hone sInueture.

关 键 词：中耳黏膜 扫描电镜 样品制备 改进 

分 类 号：R764.2[医药卫生—耳鼻咽喉科]