基于基因组序列的副溶血弧菌两种分型方法比较研究  

Comparison of the two typing methods based on the genome sequence for Vibrio parahaemolyticus

作　　者：张景山[1] 赵文轩 陈美玲 杜小莉[1] 赵林 王紫鉴 肖文静 张巍巍 赵宏群 李杰[1] 刁保卫[1] 阚飙[1] 逄波[1] Zhang Jingshan;Zhao Wenxuan;Chen Meiling;Du Xiaoli;Zhao Lin;Wang Zijian;Xiao Wenjing;Zhang Weiwei;Zhao Hongqun;Li Jie;Diao Baowei;Kan Biao;Pang Bo(National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《疾病监测》2021年第5期457-461,共5页Disease Surveillance

基　　金：国家科技重大专项(No.2018ZX10714-002);国家重点研发计划(No.2018YFC1603800)。

摘　　要：目的比较基于全基因组测序(WGS)SNP的分型方案和多位点串联重复序列分析(MLVA)分型方案用于我国副溶血弧菌分子分型的能力,并分析两种方法的优缺点及适用情况。方法选取我国分离的56株副溶血弧菌,使用6个数目可变串联重复序列(VNTR)位点的MLVA方案和基于全基因组序列SNP的方案对这些菌株进行分子分型分析,通过量化指标评价这两种方法对我国分离的副溶血弧菌的分型能力。结果56株副溶血弧菌经MLVA分型,获得31种MLVA型,D值为0.949。对56株副溶血弧菌进行全基因组测序,获得33个差异明显的基因型,D值为0.967。两种分型方法对菌株的分辨能力十分接近,分型结果高度一致。结论6位点的MLVA分型方案与基于全基因组测序SNP的分型方案具有相似的分型能力,其分型能力稍弱。Objective To compare the ability of the whole-genome sequencing(WGS)single nucleotide polymorphism(SNP)typing scheme with the multilocus tandem repeat analysis(MLVA)typing scheme for molecular typing of Vibrio parahaemolyticus isolated in China,and analyze the advantages and disadvantages of the two methods.Methods The MLVA scheme with six Variable number of tandem repeats(VNTR)loci and the SNP scheme based on the whole genome sequence were used to analyze the molecular typing of 56 strains of V.parahaemolyticus isolated in China,and evaluate the effects of these two methods with the isolates in China with quantitative indicators.Results Thirty-one MLVA types were obtained from 56 strains of V.parahaemolyticus by MLVA and the D value was 0.949.Thirty-three core genome SNP types were obtained in the same collection of strains by core genome SNP typing and the D value was 0.967.The discriminatory ability of the two typing methods is very close,and the typing results are highly consistent.Conclusion The 6-site MLVA typing scheme has similar typing capabilities to the typing scheme based on core genome SNP.

关 键 词：副溶血弧菌 多位点串联重复序列分析 数目可变串联重复序列 单核苷酸多态性 全基因组测序 分型方法 

分 类 号：R211[医药卫生—中医学] R378