Peiminine通过调控COX-2/PGE2/EGFR信号通路促进人结肠癌HCT-116细胞凋亡的分子机制  

Molecular Mechanism of Peiminine Promotes Apoptosis of Human Colon Cancer HCT-116 Cells by Regulating COX-2/PGE2/EGFR Signaling Pathway

作　　者：饶军 熊爱华 张康梅 何勤思 郑智[1] RAO Jun;XIONG Aihua;ZHANG Kangmei(Jiangxi Cancer Hospital,Nanchang,330029)

机构地区：[1]江西省肿瘤医院,南昌大学附属肿瘤医院,330029

出　　处：《实用癌症杂志》2021年第6期871-874,共4页The Practical Journal of Cancer

基　　金：国家自然科学基金项目(编号:81960865);江西省科技厅计划项目(编号:20171BBG70119、20181BAB205047);国家癌症中心攀登基金(编号:NCC201814B045);江西省卫健委课题项目(编号:20203533)。

摘　　要：目的研究Peiminine调控COX-2/PGE2/EGFR信号通路促使人结肠癌HCT-116细胞凋亡的分子机制。方法基于ELISA法、Real-time qPCR和Western Blot法测定Peiminine处理人结肠癌HCT-116细胞后PGE2、COX-1、COX-2、ERK、P-ERK、P53、P-P53、P38和P-P38含量的变化。结果与对照组(Ctrl)相比,Peiminine处理后细胞内PGE2含量显著降低(P<0.01)。同时利用Real-time qPCR和Western Blot法检测发现Peiminine能够明显降低人结肠癌HCT-116细胞COX-2的含量,IL-6和NF-κB也显著下调而IL-10明显上调。后期的研究发现EGFR信号通路中关键蛋白(ERK、P-ERK、P53、P-P53、P38和P-P38)的表达量经Peiminine处理后均降低,具有统计学差异(P<0.01)。结论Peiminine可以抑制COX-2的表达和PGE2的生成,改善抗肿瘤免疫反应,抑制EGFR信号通路,从而促进肿瘤细胞凋亡,达到抗肿瘤的作用。Objective To study the molecular mechanism that peiminine regulates COX-2/PGE2/EGFR signaling pathway and promotes the apoptosis of human colon cancer HCT-116 cells.Methods The changes of levels of PGE2,COX-1,COX-2,ERK,p-ERK,p53,p-p53,p38 and p-p38 were measured by ELISA,real-time qPCR and Western blot.Results Compared with the control group(CTRL),the content of PGE2 in the cells treated with peiminine was significantly decreased(P<0.01).At the same time,both of real-time qPCR and Western blot analyses showed that peiminine could significantly reduce the content of COX-2,down regulate IL-6 and NF-κB,but up-regulate IL-10.Further study found that the expression of key proteins(ERK,p-ERK,p53,p-p53,p38 and p-p38)in EGFR signaling pathway were decreased after peiminine treatment(P<0.01).ConclusionPeiminine could inhibit the expression of COX-2 and the production of PGE2,improve the anti-tumor immune response,inhibit the EGFR signaling pathway,promote the apoptosis of tumor cells,and achieve the anti-tumor effect.It provides a new strategy of integrated traditional Chinese and Western medicine for the treatment of patients with clinically targeted drug-resistant colon cancer.

关 键 词：贝母素乙 COX-2/PGE2/EGFR信号通路 结肠癌HCT-116细胞 凋亡 

分 类 号：R735.35[医药卫生—肿瘤]