Mn^(2+)掺杂普鲁士蓝纳米颗粒T1-T2双模磁共振成像和光热治疗的体外研究  

Mn^(2+)-doped Prussian blue nanoparticles for T1-T2 dual-mode magnetic resonance imaging and photothermal therapy in vitro

作　　者：何庚翰 陶泉 刘闯 张迪 周宇平 刘瑞源[1] HE Genghan;TAO Quan;LIU Chuang;ZHANG Di;ZHOU Yuping;LIU Ruiyuan(School of Biomedical Engineering,Southern Medical University,Guangzhou,510515 China)

机构地区：[1]南方医科大学生物医学工程学院,广东广州510515

出　　处：《南方医科大学学报》2021年第6期909-915,共7页Journal of Southern Medical University

基　　金：国家自然科学基金(81671749)。

摘　　要：目的制备一种Mn^(2+)掺杂普鲁士蓝纳米颗粒,用于检测体外T1-T2双模磁共振成像和光热治疗效果。方法采用微乳液法,以二氯化锰、氯化亚铁和铁氰化钾为原料制备Mn^(2+)掺杂普鲁士蓝纳米颗粒;观察纳米颗粒的体外T1-T2双模磁共振成像性能;考察808 nm激光照射后纳米颗粒的升温效果;通过CCK-8法和AM/PI活死细胞染色法观察体外光热治疗效果。结果成功制备了Mn^(2+)掺杂普鲁士蓝纳米颗粒,粒径为39.46±0.42 nm,电位为-25.9±1.2 mV,粒径均匀,分散性好;808 nm激光照射10 min后,纳米颗粒分散系可以升温至90℃。由7T磁共振测得的数据拟合获得r1值为0.68(mmol/L)^(-1)s^(-1),r2值为3.65(mmol/L)^(-1)s^(-1),具有良好的T1-T2双模磁共振成像效果;在808 nm激光照射下,可显著降低细胞存活率(P<0.05);CCK-8法和AM/PI活死细胞染色法的结果均显示纳米颗粒对HepG2细胞具有良好的光热治疗效果。结论成功制备了粒径均一的Mn^(2+)掺杂普鲁士蓝纳米颗粒,该纳米颗粒的T1-T2双模磁共振成像效果和体外光热治疗效果良好,且无明显细胞毒性。Objective To prepare Mn^(2+)-doped Prussian blue nanoparticles(Mn-PB NPs)for T1-T2 dual-mode magnetic reso nance imaging(MRI)and photothermal therapy in vitro.Methods Mn-PB NPs were prepared based on manganese chloride,ferrous chloride and potassium ferricyanide using the microemulsion method.The performance of T1-T2 dual-mode MRI with Mn-PB NPs and the photothermal property of the nanoparticles were assessed.CCK-8 assay and AM/PI double staining were used to evaluate the effect of photothermal therapy in vitro using the parepared nanoparticles.Results The prepared Mn-PB NPs had a mean particle size of 39.46±0.42 nm with a Zeta potential of-25.9±1.2 mV and exhibited a good dispersibility and uniform particle size.In MRI using the nanoparticles,the r1 and r2 values reached 0.68 and 3.65(mmol/L)^(-1)s^(-1),respectively,indicating good performance of Mn-PB NPs for T1 and T2 enhancement in MRI.When irradiated with 808 nm laser for 10 min,Mn-PB NPs showed a temperature rise to 90℃to cause significant reduction of cell survival.CCK-8 assay and AM/PI double staining confirmed that Mn-PB NPs were capable of efficient killing of HepG2 cells upon 808 nm laser irradiation.Conclusion The Mn-PB NPs prepared in this work have uniform particle size and show good performances both in MRI for T1 and T2 enhancement and in photothermal therapy in vitro without obvious cytotoxicity.

关 键 词：普鲁士蓝 微乳液 锰掺杂 磁共振成像 光热治疗 

分 类 号：R730.5[医药卫生—肿瘤]