散结抑癌方联合贝伐珠单抗对BALB/c裸鼠结肠癌肺转移的影响  

Effect of San-Jie-Yi-Ai Decoction combined with bevacizumab on lung metastasis of colon cancer in BALB/c nude mice

作　　者：张莹瑄 任建琳[1] 吴杏黎 陆鑫熠 黄人可 ZHANG Yingxuan;REN Jianlin;WU Xingli;LU Xinyi;HUANG Renke(Department of Oncology,Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]上海中医药大学附属市中医医院肿瘤科,200071

出　　处：《国际消化病杂志》2021年第3期200-204,229,共6页International Journal of Digestive Diseases

基　　金：国家自然科学基金项目(81873279);上海市卫生健康委员会卫生行业临床研究专项(201840325);上海市科学技术委员会医学引导类(中、西医)科技支撑项目(19401935200);上海申康医院发展中心临床三年行动计划项目(SHDC2020CR2047B);2019年上海中医药大学研究生创新培养专项(Y2020074)。

摘　　要：目的探究散结抑癌方联合贝伐珠单抗对BALB/c裸鼠结肠癌肺转移的影响,以及对血管内皮生长因子(VEGF)蛋白表达的影响。方法采用经尾静脉注射对数生长期的人源结肠癌HCT116荧光细胞的方法,构建BALB/c裸鼠肺转移模型。将造模成功的20只裸鼠随机分为4组,每组5只。中药组,散结抑癌方灌胃;西药组,贝伐珠单抗腹腔注射;联合组,散结抑癌方灌胃+贝伐珠单抗腹腔注射;对照组,生理盐水灌胃。每周进行1次活体荧光成像观察肿瘤生长情况,记录小鼠体质量。干预4周后取小鼠肺组织,H-E染色观察小鼠结肠癌肺转移情况,免疫组织化学法检测VEGF蛋白表达情况。结果与干预前比较,对照组在干预后体质量下降最多。干预后,中药组体质量略高于对照组(P>0.05),联合组体质量略高于西药组(P>0.05),联合组和西药组体质量均明显高于对照组(P<0.05)。小动物成像定量分析结果显示,干预后中药组、西药组和联合组生物发光光子数均少于对照组(P均<0.05)。H-E染色结果指出,对照组转移灶面积最大,转移处最多,联合组转移灶面积最小,转移处最少。免疫组织化学检测结果显示,对照组VEGF蛋白平均阳性细胞率为48.57%,高于中药组(39.91%)、西药组(35.82%)和联合组(29.03%),差异均有统计学意义(P均<0.01)。结论散结抑癌方可以抑制结肠癌肺转移,作用途径可能与VEGF蛋白表达相关。Objective This paper aims to explore the effect of San-Jie-Yi-Ai Decoction combined with bevacizumab on lung metastasis of colon cancer and the expression of vascular endothelial growth factor(VEGF)in BALB/c nude mice.Methods BALB/c nude mice models of lung metastasis were established by injecting human colon cancer HCT116 fluorescent cells in logarithmic growth phase via tail vein.Twenty nude mice successfully modeled were randomly divided into 4 groups with 5 mice in each group.In the traditional Chinese medicine group,San-Jie-Yi-Ai Decoction was given by gavage.In the western medicine group,bevacizumab was injected intraperitoneally.In the combined group,San-Jie-Yi-Ai Decoction was given by gavage and bevacizumab was injected intraperitoneally.The control group was given normal saline by gavage.The tumor growth was observed by in vivo fluorescence imaging once a week,and the body weight of mice was recorded.After 4 weeks of intervention,the lung tissues of mice were taken,the lung metastasis of colon cancer was observed by H-E staining,and the expression of VEGF protein was detected by immunohistochemistry.Results Compared with before the intervention,the weight of nude mice in the control group decreased the most.After the intervention,the weight of nude mice in the traditional Chinese medicine group was slightly higher than that in the control group(P>0.05),the weight of nude mice in the combined group was slightly higher than that in the western medicine group(P>0.05),and the weight of nude mice in the two groups were significantly higher than that in the control group(P<0.05).The results of quantitative analysis of small animal imaging showed that the number of bioluminescence photons in the traditional Chinese medicine group the western medicine group,and the combined group were less than those in the control group after intervention(P<0.05).The H-E staining showed that the control group had the largest area of metastases and the largest number of metastases,while the combined group had the smallest area

关 键 词：散结抑癌方 结肠癌肺转移 贝伐珠单抗 血管内皮生长因子 

分 类 号：R73[医药卫生—肿瘤]