裸鼠异种移植急性T淋巴细胞白血病模型的建立  

Stablishment of acute T-lymphocyte leukemia model by xenotransplantation from nude mice

作　　者：张爱君 潘文文 胡晓曦 吴育晶[1] 魏伟[1] Zhang Aijun;Pan Wenwen;Hu Xiaoxi(Institute of Clinical Pharmacology,Anhui Medical University,Key Laboratory of Anti-inflammatory and Immune Medicine,Ministry of Education,Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine,Hefei 230032)

机构地区：[1]安徽医科大学临床药理研究所,抗炎免疫药物教育部重点实验室,抗炎免疫药物安徽省协同创新中心,合肥230032

出　　处：《安徽医科大学学报》2021年第6期908-914,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81973332、81603121)。

摘　　要：目的比较尾静脉注射和皮下注射人Jurkat细胞建立异种移植急性T淋巴细胞白血病模型的优缺点。方法雌性BALB/c裸鼠随机分为正常组、尾静脉移植组和皮下移植组。除正常组外,其余裸鼠造模前连续2 d给予环磷酰胺(2 mg/只),尾静脉移植组连续2 d尾静脉接种5×106个Jurkat细胞,皮下移植组连续2 d皮下接种5×106个Jurkat细胞,正常组裸鼠尾静脉注射相同体积PBS缓冲液。观察并记录裸鼠体质量、弓背以及精神状态。第7天和第14天通过流式细胞术检测Jurkat细胞在裸鼠外周血中浸润情况。第28天待尾静脉移植组和皮下移植组裸鼠出现衰竭体征濒临死亡时处死裸鼠,记录裸鼠体质量,瑞氏-吉姆萨染色检测外周血和骨髓中Jurkat细胞的浸润情况,流式细胞术检测脾脏和骨髓中Jurkat细胞的百分比,HE染色、免疫组化法检测肝脏和脾脏中浸润的Jurkat细胞表达,ELISA检测血清中Jurkat细胞分泌IL-2水平。结果与正常组相比,尾静脉移植组和皮下移植组裸鼠均逐渐出现体质量下降、弓背、食欲减退等表现,肝脏、脾脏及骨髓中均有人Jurkat细胞表达。尾静脉移植组成模率73%,皮下移植组成模率40%。结论尾静脉注射和皮下注射异种移植Jurkat细胞两种方法均可成功建立急性T淋巴细胞白血病动物模型,尾静脉注射移植效果更佳。Objective To compare the advantages and disadvantages between caudal vein injection and subcutaneous injection of human Jurkat cells in the establishment of xenograft acute T lymphocytic leukemia model.Methods Female BALB/c nude mice were randomly divided into normal group,tail vein transplantation group and subcutaneous transplantation group.The tail vein transplantation group and subcutaneous transplantation groups were given cyclophosphamide for 2 consecutive days before modeling.5×106 Jurkat cells were inoculated via tail vein in tail vein transplantation group for 2 consecutive days,and 5×106 Jurkat cells were subcutaneously injected into subcutaneous transplantation group for 2 consecutive days.The same volume of phosphate buffer saline was injected into the normal group by tail vein.The body weight,bow back and mental state of nude mice were observed and recorded.The percentages of Jurkat cells in peripheral blood were monitored by flow cytometry on the 7th and 14th day.On the 28th day,the mice appeared signs of failure and were close to death,and they were put to death.At the same time,the body weight was recorded.Wright′s stain was used to detect the infiltration of Jurkat cells in peripheral blood and bone marrow,spleen and bone marrow of the Jurkat invasion was detected by flow cytometry.Pathology of liver and spleen was also observed by HE staining,and infiltration of Jurkat cells was observed by immunohistochemistry.The level of IL-2 secreted by Jurkat cells in serum was detected by ELISA.Results Compared with the normal group,the tail vein transplantation group and subcutaneous transplantation groups nude mice gradually showed weight loss,bow back and loss of appetite,and Jurkat cells were expressed in the liver,spleen and bone marrow of the mice in each group.The success rate of tail vein transplantation group was 73%,and the success rate of subcutaneous transplantation group was 40%.Conclusion Both tail vein injection and subcutaneous injection of human Jurkat cells can successfully establish

关 键 词：急性T淋巴细胞白血病细胞 BALB/C裸鼠 异种移植 动物模型 尾静脉注射 皮下注射 

分 类 号：R73.35[医药卫生—肿瘤] R73.352[医药卫生—临床医学]