雷公藤甲素对急性高眼压大鼠视网膜神经节细胞的保护作用  

Protective effect of triptolide on retinal ganglion cells in rats with acute ocular hypertension

作　　者：汪新芳 马滕 WANG Xinfang;MA Teng(Department of Ophthalmology,Beijing University of Chinese Medicine Zaozhuang Hospital,Shandong Province,Zaozhuang 277000,China)

机构地区：[1]山东省枣庄市中医医院北京中医药大学枣庄医院眼科,山东枣庄277000

出　　处：《中国医药导报》2021年第18期13-16,F0004,共5页China Medical Herald

基　　金：山东省中医药管理局中医药科技发展计划项目(2019-0642)。

摘　　要：目的观察雷公藤甲素对急性高眼压大鼠视网膜神经节细胞(RGCs)的保护作用。方法选取60只Wister大鼠,雌雄各半,采用随机数字表法将其分为对照组、模型组、雷公藤甲素组,每组20只。模型组、雷公藤甲素组通过前房灌注加压法建模,建模成功后,雷公藤甲素组每日腹腔注射雷公藤甲素10μg/kg,对照组、模型组每日腹腔注射等量的生理盐水,三组共注射7 d。采用HE染色观察三组视网膜病理学改变;TUNEL法染色观察RGCs凋亡情况,免疫荧光法观察其存活情况;免疫组化检测三组视网膜中Caspase-3表达情况。结果对照组视网膜各层次排列规整,细胞核边界清楚。模型组视网膜各层次排列不规整、视网膜萎缩,而雷公藤甲素组视网膜各层次清晰,排列整齐。模型组视网膜神经纤维层(RNFL)厚度值、凋亡RGCs数及Caspase-3阳性细胞数高于对照组,存活RGCs数低于对照组,差异有统计学意义(均P<0.05)。雷公藤甲素组RNFL厚度值、凋亡RGCs数及Caspase-3阳性细胞数低于模型组,存活RGCs数高于模型组,差异有统计学意义(均P<0.05)。结论雷公藤甲素对急性高眼压大鼠RGCs具有明显保护作用。Objective To observe the protective effect of triptolide on retinal ganglion cells(RGCs)in rats with acute ocular hypertension.Methods Sixty Wister rats were selected,male and female in half.The rats were divided into control group,model group and Triptolide group by random number table method,with 20 rats in each group.Model group and Triptolide group were modeled by anterior chamber perfusion and pressure method.After successful modeling,the Triptolide group was intraperitoneally injected with 10μg/kg of Triptolide daily,while the control group and model group were intraperitoneally injected with the same amount of normal saline daily,for a total of seven days.The pathological changes of retinal layers were observed by HE staining.The apoptosis of RGCs was observed by TUNEL staining and the survival was observed by immunofluorescence method.The expression of caspase-3 in the retinas of the three groups was detected by immunohistochemistry.Results In the control group,the retinal layers were arranged neatly and the nuclear boundaries were clear.The retinal layers of the model group were irregular and atrophied,while the retinal layers of the triptolide group were clear and orderly.The thickness of retinal nerve fiber layer(RNFL),the number of apoptotic RGCs and the number of caspase-3 positive cells in model group were higher than those in control group,and the number of survival RGCs was lower than that in control group,with statistical significance(all P<0.05).The thickness of RNFL,number of apoptotic RGCs and number of caspase-3 positive cells in triptolide group were lower than those in model group,and the number of survival RGCs was higher than that in model group,with statistical significance(all P<0.05).Conclusion Triptolide has a significant protective effect on RGCs in rats with acute ocular hypertension.

关 键 词：雷公藤甲素 青光眼 急性高眼压 视网膜神经节细胞 

分 类 号：R775[医药卫生—眼科]