Genome assembly and transcriptome analysis provide insights into the antischistosome mechanism of Microtus fortis  

作　　者：Hong Li Zhen Wang Shumei Chai Xiong Bai Guohui Ding Yuanyuan Li Junyi Li Qingyu Xiao Benpeng Miao Weili Lin Jie Feng Mingyue Huang Cheng Gao Bin Li Wei Hu Jiaojiao Lin Zhiqiang Fu Jianyun Xie Yixue Li 

机构地区：[1]Bio-Med Big Data Center,CAS Key Laboratory of Computational Biology,Shanghai Institute of Nutrition and Health,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai 200031,China [2]Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Animal Parasitology,Ministry of Agriculture,Shanghai 200241,China [3]Shanghai Laboratory Animal Research Center,Shanghai 201203,China [4]Institute for Digital Health,International Human Phenome Institutes(Shanghai),Shanghai 200433,China [5]Shanghai Center for Bioinformation Technology,Shanghai Academy of Science and Technology,Shanghai 201203,China [6]School of Computer Science and Technology,Harbin Institute of Technology(Shenzhen),Shenzhen,Guangdong 518055,China [7]Shanghai Institute of Immunology,Department of Immunology and Microbiology,Shanghai JiaoTong University School of Medicine,Shanghai 200025,China [8]State Key Laboratory of Genetic Engineering,Ministry of Education Key Laboratory of Contemporary Anthropology,Collaborative Innovation Center for Genetics and Development,School of Life Sciences,Fudan University,Shanghai 200438,China [9]Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou,Zhejiang 330106,China

出　　处：《Journal of Genetics and Genomics》2020年第12期743-755,共13页遗传学报（英文版）

基　　金：This work was supported by the Key Project in the National Science&Technology Pillar Program from the Ministry of Science and Technology(2015BAI09B04);the National Natural Science Foundation of China(31872256,31472188);the National Key Research and Development Program of China(2017YFD0501306);the Science and Technology Service Network Initiative of Chinese Academy of Sciences(KFJ-STS-QYZD-126,ZDBS-SSW-DQC-02);CAS Youth Innovation Promotion Association,and SA-SIBS Scholarship Program.

摘　　要：Microtus fortis is the only mammalian host that exhibits intrinsic resistance against Schistosoma japonicum infection.However,the underlying molecular mechanisms of this resistance are not yet known.Here,we perform the first de novo genome assembly of M.fortis,comprehensive gene annotation analysis,and evolution analysis.Furthermore,we compare the recovery rate of schistosomes,pathological changes,and liver transcriptomes between M.fortis and mice at different time points after infection.We observe that the time and type of immune response in M.fortis are different from those in mice.M.fortis activates immune and inflammatory responses on the 10th day post infection,such as leukocyte extravasation,antibody activation,Fc-gamma receptor-mediated phagocytosis,and the interferon signaling cascade,which play important roles in preventing the development of schistosomes.In contrast,an intense immune response occurrs in mice at the late stages of infection and could not eliminate schistosomes.Infected mice suffer severe pathological injury and continuous decreases in cell cycle,lipid metabolism,and other functions.Our findings offer new insights into the intrinsic resistance mechanism of M.fortis against schistosome infection.The genome sequence also provides the basis for future studies of other important traits in M.fortis.

关 键 词：Genome assembly Microtus fortis SCHISTOSOME IMMUNE TRANSCRIPTOME 

分 类 号：R532.21[医药卫生—内科学]