外植体再生分子调控机理  

Molecular regulatory mechanisms of explant regeneration

作　　者：李俊[1] 范世航 刘婧琳 刘静 华玮[1] LI Jun;FAN Shi-hang;LIU Jing-lin;LIU Jing;HUA Wei(Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2021年第3期376-382,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31100236);国家重点研发计划项目(2016YFD0100500)。

摘　　要：植物细胞全能性被认为是植物细胞工程的基础。不同类型的外植体,受创伤刺激诱导器官再生和体细胞胚胎发生,可形成完整植株,实现无性繁殖。外植体再生高度依赖发育和环境信号。本文概述了外植体对创伤信号的感知和重编程启动、芽和根器官发生过程中的外源激素平衡和基因表达、体细胞胚胎发生的分子调控和内源激素的动态变化等内容,特别概述了外植体再生重编程过程中的表观调控机制研究进展,旨在为获得高效的再生体系、提高遗传转化效率提供理论支撑。Plant cell totipotency is considered to be the fundamental basis for plant cell engineering.Different explants could regenerate into intact plants to fulfill asexual reproduction via de novo organogenesis or somatic embryogenesis induced by wounding stimulus.Actually,explants regeneration is highly depended on developmental and environmental signals.This paper reviewed explants perception of wounding signal and reprogramming initiation,shoot and root regeneration under exogenous auxin/cytokinin balance and gene expression,somatic embryogenesis regulation and hormone dynamics,especially the epigenetic regulation during the process of reprogramming in explant regeneration.It was expected to obtain high efficient explant regeneration system for genetic transformation and production practice.

关 键 词：细胞全能性 再生 器官发生 体细胞胚胎发生 重编程 

分 类 号：Q813.1[生物学—生物工程]