结合PCA和GWAS解析甘蓝型油菜株型相关性状的遗传调控位点  

Genetic dissection of plant architecture-related traits by GWAS with PCA in Brassica napus

作　　者：张旭[1] Luqman Bin Safdar 唐敏强 刘越英[2] 张园园[2] 刘胜毅[2] ZHANG Xu;Safdar Luqman Bin;TANG Min-qiang;LIU Yue-ying;ZHANG Yuan-yuan;LIU Sheng-yi(School of Mathematics and Statistics,Southwest University,Chongqing 400715,China;Oil Crops Research In-stitute,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China)

机构地区：[1]西南大学数学与统计学院,重庆400715 [2]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2021年第3期462-469,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(11701471);重庆市基础科学与前沿技术研究(cstc2017jcyjAX0476);国家自然科学基金青年科学基金(31801730);中国博士后科学基金面上项目(2016M601188);中国博士后科学基金(2018T110166)。

摘　　要：作物株型改良是提高收获指数和产量的重要途径,为了解其复杂的遗传调控机制,以373份甘蓝型油菜组成的自然群体为基础,对4个株型相关性状(株高、第一分枝高度、有效分枝数和主花序长度)进行多环境下表型鉴定,组合利用全基因组关联分析(GWAS)和主成分分析(PCA)方法对株型的遗传调控进行解析。研究表明,PCA可以合理地解释株型的相关表型,基于主成分的GWAS和基于单个性状的GWAS的结果可互相验证和互为补充,挖掘更多的调控位点和信息。进一步,从染色体A01、A10和C06中筛选到19个株型相关的候选基因,其中,位于染色体C06上的两个候选基因跟PC1-GWAS鉴定到的新位点相关。此方法和结果为株型等复杂性状的形成机制解析提供了新的思路和策略。For global demand of high yield breeding,complex genetic regulation on plant architecture was focused by genome-wide association study(GWAS)and principal component analysis(PCA),based on 4 typical traits related to plant architecture(PA)of 373 B.napus(L.)accessions.The 4 traits included plant height,branch height,branch number and main inflorescence length in 3 environments(Wuhan 2014-2015,Wuhan 2015-2016,and Yangzhou 2015-2016).Results showed that PCA could reasonably explain the phenotypes related to PA.PCA GWAS and normal GWAS could verify and complement each other and provide the most information on traits that determine rapeseed architecture.Further,19 PA related candidate genes were obtained from chromosomes A01,A10 and C06.Two genes on chromosome C06 were related to the new loci identified by PC1-GWAS.This method and results provide new ideas and strategies for the analysis of the formation mechanism of complex traits such as PA.

关 键 词：植物株型 甘蓝型油菜 主成分分析 全基因组关联分析 

分 类 号：S565.4[农业科学—作物学]