薤中新的甾体皂苷类化学成分  

Chemical Constituents of New Steroidal Saponins from Allium chinense G. Don

作　　者：杨依然 姚华 闫江红[3] 孙志恒 张余 房雪晴 李绪文[1] 金永日[1] YANG Yiran;YAO Hua;YAN Jianghong;SUN Zhiheng;ZHANG Yu;FANG Xueqing;LI Xuwen;JIN Yon-Ri(College of Chemistry,Jilin University,Changchun 130012,China;China-Japan Union Hospital of Jilin University,Changchun 130033,China;The First Clinical Hospital of Jilin Academy of Traditional Chinese Medicine,Changchun 130021,China;School of Material Science,University of New South Wales,Sydney 2033,Australia)

机构地区：[1]吉林大学化学学院,长春130012 [2]吉林大学中日联谊医院,长春130033 [3]吉林省中医药科学院第一临床医院,长春130021 [4]新南威尔士大学材料科学学院,悉尼2033,澳大利亚

出　　处：《高等学校化学学报》2021年第6期1742-1753,共12页Chemical Journal of Chinese Universities

基　　金：吉林省科技发展计划项目(批准号:20200404138YY)资助。

摘　　要：从薤(Allium chinense G.Don)的乙醇提取物中分离得到6个新甾体皂苷类化合物,通过波谱数据及理化性质分析,鉴定其分别为5α-cholano-22,16-内酯-3-O-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃半乳糖苷(1)、6-酮-5α-cholano-22,16-内酯-3-O-β-D-吡喃木糖基-(1→4)-[α-L-吡喃阿拉伯糖基-(1→6)]-β-D-吡喃葡萄糖苷(2)、(25R)-26-O-β-D-吡喃葡萄糖基-5α-呋喃甾烷-3β,26-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃半乳糖苷(3)、(25R)-6-酮-26-O-β-D-吡喃葡萄糖基-5α-呋喃甾烷-3β,22α,26-三醇-3-O-α-L-吡喃木糖基-(1→4)-β-D-吡喃葡萄糖苷(4)、(25R)-6-酮-5α-呋喃甾烷-3β,22α,24β,26-四醇-3-O-β-D-吡喃木糖基-(1→4)-[α-L-吡喃阿拉伯糖基-(1→6)]-β-D-吡喃葡萄糖苷(5)和(25R)-5α-呋甾-2α,3β,22α,26-四醇-26-O-β-D-吡喃葡萄糖苷(6).化合物1和2的皂苷元骨架在天然产物中首次分离得到.选用H_(2)O_(2)诱导PC12细胞神经氧化损伤模型,初步考察了6种新的呋甾型化合物的抗氧化活性,实验结果表明,化合物3对由H_(2)O_(2)诱导的细胞氧化损伤有显著的保护效果.Six new steroidal saponins were isolated from 98% ethanol extract of Allium chinense G. Don.Through spectral data as well as physical and chemical analysis,they were identified as 5α-cholano-22,16-lactone-3-O-β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galacopyranoside(1),6-ketone-5α-cholano-22,16-lactone-3-O-β-D-6-xylopyranosyl-(1→4)-[α-L-arabinopyranosyl-(1→6)]-β-D-glucopyranoside(2),(25R)-26-O-β-D-glucopyranosyl-5α-furostane-3β,26-diol-3-O-β-Dglucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galacopyranoside(3),(25R)-6-ketone-26-O-β-D-glucopyranosyl-5α-furostane-3β,22α,26-triol-3-O-α-L-xylopyranosyl-(1→4)-β-D-glucopyranoside(4),(25R)-6-ketone-5α-furostane-3β,22α,24β,26-tetraol-3-O-β-D-xylopyranosyl-(1→4)-[α-L-arabinopyranosyl-(1→6)]-β-D-glucopyranoside(5)and(25R)-5α-furostane-2α,3β,22α,26-tetraol-26-O-β-D-glucopyranoside(6). To the best of our knowledges,the sapogenin skeletons of compound 1 and compound 2 were isolated from natural products for the first time. The cell proliferation-toxicity test kit(CCK-8)was used to explore the PC12 cells hydrogen peroxide induced the protective effect of oxidative damage. The experimental results showed that compound 3 had a significant protective effect on cell oxidative damage induced by hydrogen peroxide.

关 键 词：薤 呋甾皂苷 结构鉴定 细胞活性 抗氧化能力 

分 类 号：O629[理学—有机化学]