微小RNA-126对胃癌细胞增殖、侵袭和转移能力的影响及其机制  

Effect of microRNA-126 on proliferation,invasion and metastasis capacities of gastric cancer cells and its mechanism

作　　者：程若溪 赖铭裕[1] 蒋莉萍[1] 吴谢慧 梁凯 CHENG Ruo-xi;LAI Ming-yu;JIANG Li-ping;WU Xie-hui;LIANG Kai(Department of Geriatric Gastroenterology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院老年消化内科,南宁市530021

出　　处：《广西医学》2021年第8期977-983,共7页Guangxi Medical Journal

基　　金：广西医疗卫生适宜技术开发与推广应用(S2019100)。

摘　　要：目的探讨微小RNA(miR)-126对胃癌细胞增殖、侵袭和转移能力的影响及其可能机制。方法将胃癌细胞株SGC-7901分为miR-126过表达组(miR-126-mimic组)、miR-126过表达阴性对照组(miR-126-mimic-NC组)、miR-126沉默组(miR-126-inhibitor组)、miR-126沉默阴性对照组(miR-126-inhibitor-NC组),转染相应慢病毒,检测各组细胞miR-126的表达情况。采用CCK-8试验、平板克隆实验检测miR-126对SGC-7901细胞增殖的影响,采用划痕实验、Transwell实验分别检测miR-126对SGC-7901迁移及侵袭能力的影响,并检测各组细胞中高尔基体磷蛋白3(GOLPH3)、磷脂酰肌醇-3激酶调控亚基2(PI3KR2)、蛋白激酶B 1(Akt1)、哺乳动物雷帕霉素靶蛋白(mTOR)的mRNA及蛋白表达水平,以及磷酸化Akt1(p-Akt1)磷酸化mTOR(p-mTOR)的蛋白表达水平。结果(1)miR-126-mimic组miR-126的表达水平高于miR-126-mimic-NC组,miR-126-inhibitor组miR-126的表达水平低于miR-126-inhibitor-NC组(均P<0.05)。(2)24 h、48 h、72 h时,miR-126-mimic组的吸光度值、愈合率均低于miR-126-mimic-NC组,克隆数、迁移细胞数、穿膜细胞数少于miR-126-mimic-NC组,而miR-126-inhibitor组以上指标高于或多于miR-126-inhibitor-NC组(均P<0.05)。(3)miR-126-mimic组GOLPH3、PI3KR2、Akt1、mTOR的蛋白及mRNA表达水平,以及p-Akt1、p-mTOR的蛋白表达水平均低于miR-126-mimic-NC组,而miR-126-inhibitor组以上指标均高于miR-126-inhibitor-NC组(均P<0.05)。结论miR-126可通过GOLPH3靶向调控PI3K/Akt/mTOR通路,从而影响胃癌细胞的增殖、侵袭及转移能力。Objective To investigate the effect of microRNA(miR)-126 on the proliferation,invasion and metastasis capacities of gastric cancer cells and its possible mechanism.Methods Gastric cancer cell lines SGC-7901 were divided into miR-126 overexpression group(miR-126-mimic group),miR-126 overexpression negative control group(miR-126-mimic-NC group),miR-126 silence group(miR-126-inhibitor group)and miR-126 silence negative control group(miR-126-inhibitor-NC group),then were transfected by corresponding lentivirus.The expression of miR-126 was detected in each group.The effect of miR-126 on SGC-7901 cell proliferation was determined by CCK-8 assay and colony formation assay,the effect of miR-126 on SGC-7901 cell metastasis and invasion capacities was detected by wound-healing assay and Transwell assay respectively;besides,the mRNA and protein expression levels of Golgi phosphoprotein 3(GOLPH3),phosphoinositide 3-kinase regulatory subunit 2(PI3KR2),protein kinase B 1(Akt1)and mammalian target of rapamycin(mTOR),and the protein expression levels of phosphorylated Akt1(p-Akt1)and phosphorylated mTOR(p-mTOR)were measured in the cells of each group.Results(1)The miR-126 expression level was higher in the miR-126-mimic group than in the miR-126-mimic-NC group,whereas was lower in the miR-126-inhibitor group than in the miR-126-inhibitor-NC group(all P<0.05).(2)At the time points of 24 h,48 h or 72 h,the miR-126-mimic group possessed a lower A value and healing rate,and less cloning number,migrating cells number and permeated cells number as compared with the miR-126-mimic-NC group,while the aforementioned indexes in the miR-126-inhibitor group were higher or more than those in the miR-126-inhibitor-NC group(all P<0.05).(3)The miR-126-mimic group yielded lower protein and mRNA expression levels of GOLPH3,PI3KR2,Akt1 and mTOR,and decreased protein expression levels of p-Akt1 and p-mTOR as compared with the miR-126-mimic-NC group,whereas the aforementioned indexes in the miR-126-inhibitor group were higher than those in the miR-1

关 键 词：胃癌 微小RNA-126 高尔基体磷蛋白3 磷脂酰肌醇-3激酶/蛋白激酶B1/哺乳动物雷帕霉素靶蛋白通路 增殖 侵袭 转移 体外实验 

分 类 号：R735.2[医药卫生—肿瘤]