Crumbs proteins stabilize the cone mosaics of photoreceptors and improve vision in zebrafish  

作　　者：Qinlong Hao Mingjie Zheng Kechao Weng Yumei Hao Yao Zhou Yuchen Lin Feng Gao Ziqi Kou Shoji Kawamura Ke Yao Pinglong Xu Jinghai Chen Jian Zou 

机构地区：[1]Eye Center of the Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China [2]The Institute of Translational Medicine,Zhejiang University,Hangzhou 310058,China [3]Department of Cardiology,The Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China [4]Department of Integrated Biosciences,Graduate School of Frontier Sciences,University of Tokyo,Kashiwa,Chiba 277-8562,Japan [5]Zhejiang Provincial Key Lab of Ophthalmology,Hangzhou 310058,China [6]MOE Laboratory of Biosystems Homeostasis&Protection and Innovation Center for Cell Signaling Network,Life Sciences Institute,Zhejiang University,Hangzhou 310058,China

出　　处：《Journal of Genetics and Genomics》2021年第1期52-62,共11页遗传学报（英文版）

基　　金：supported by National Natural Science Foundation of China projects (81770938 to J.Z. and 81570822 to K.Y.);the Zhejiang Provincial Natural Science Foundation project (LZ15H120001 to J.Z.);the Fundamental Research Funds for the Central Universities (2016FZA7010 and 2017FZA7001 to J.Z.)。

摘　　要：Although the unique organization of vertebrate cone mosaics was first described long ago,both their underlying molecular basis and physiological significance are largely unknown.Here,we demonstrate that Crumbs proteins,the key regulators of epithelial apical polarity,establish the planar cellular polarity of photoreceptors in zebrafish.Via heterophilic Crb2a-Crb2b interactions,the apicobasal polarity protein Crb2b restricts the asymmetric planar distribution of Crb2a in photoreceptors.The planar polarized Crumbs proteins thus balance intercellular adhesions and tension between photoreceptors,thereby stabilizing the geometric organization of cone mosaics.Notably,loss of Crb2b in zebrafish induces a nearsightedness-like phenotype in zebrafish accompanied by an elongated eye axis and impairs zebrafish visual perception for predation.These data reveal a detailed mechanism for cone mosaic homeostasis via previously undiscovered apical-planar polarity coordination and propose a pathogenic mechanism for nearsightedness.

关 键 词：Crumbs proteins Apical polarity Planar cellular polarity Cone mosaics Nearsightedness Visual perception 

分 类 号：R-332[医药卫生] R778.11