Improving the Cpf1-mediated base editing system by combining dCas9/dead sgRNA with human APOBEC3A variants  

作　　者：Meng Lian Fangbing Chen Xingyun Huang Xiaozhu Zhao Shixue Gou Nan Li Qin Jin Hui Shi Yanhui Liang Jingke Xie Weikai Ge Zhenpeng Zhuang Jiaowei Wang Yinghua Ye Yi Yang Kepin Wang Liangxue Lai Han Wu 

机构地区：[1]CAS Key Laboratory of Regenerative Biology,Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510530,China [2]Institute of Physical Science and Information Technology,Anhui University,Hefei 230601,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]Research Unit of Generation of Large Animal Disease Models,Chinese Academy of Medical Sciences(2019RU015),Guangzhou 510530,China [5]Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory),Guangzhou 510005,China [6]Key Laboratory for Major Obstetrics Diseases of Guangdong Province,Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes,The Third Hospital Affiliated of Guangzhou Medical University,Guangzhou 510150,China

出　　处：《Journal of Genetics and Genomics》2021年第1期92-95,共4页遗传学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31901048);the National Key Research and Development Program of China (2017YFA0105103, 2019YFA0111500);the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16030503);Key Research & Development Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory (2018GZR110104004);the Youth Innovation Promotion Association of the Chinese Academy of Sciences (2019347);Science and Technology Planning Project of Guangdong Province, China (2019A1515012090, 2017B020231001, 2017B030314056, 2014B020225003);Science and Technology Program of Guangzhou, China (201704030034, 202007030003, 202002030382);Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019-I2M-5-025)。

摘　　要：Base editor-mediated C-to-T base conversion obviates the requirements of double-strand breaks,thereby showing promise as a tool for disease modeling and gene therapy(Gaudelli et al.,2017;Rees and Liu 2018).The most actively used base editor comprises a Cas9 nickase(nCas9)with cytidine deaminase and fused uracil DNA glycosylase inhibitor at the carboxy terminus of nCas9 to inhibit uracil N-glycosylase effects(Pearl,2000;Kunz et al.,2009;Rees and Liu 2018).

关 键 词：BASE URACIL thereby 

分 类 号：Q78[生物学—分子生物学]