不同来源耐甲氧西林金黄色葡萄球菌的耐药调查与优势基因型分析  

Antimicrobial Resistance Investigation and Dominant Genotype Analysis of Methicillin-resistant Staphylococcus aureus from Different Sources

作　　者：许弘仪 辛宇航 刘娜[2] 张青青 王娟[2] 李月华[2] 黄秀梅[2] 王龙光[1,2] 曲志娜[2] 刘焕奇[1] XU Hong-yi;XIN Yu-hang;LIU Na;ZHANG Qing-qing;WANG Juan;LI Yue-hua;HUANG Xiu-mei;WANG Long-guang;QU Zhi-na;LIU Huan-qi(College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China;China Animal Health and Epidemiology Center,Qingdao 266032,China)

机构地区：[1]青岛农业大学动物医学院,山东青岛266109 [2]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《中国兽医杂志》2021年第2期20-26,F0002,共8页Chinese Journal of Veterinary Medicine

基　　金：山东省现代农业产业技术体系创新团队项目(SDAIT-09-03);国家重点研发计划项目(2017YFD5010406)。

摘　　要：为了解国内不同来源耐甲氧西林金黄色葡萄球菌(MRSA)的流行分布及耐药特征,分析其优势基因型,为有效控制MRSA流行与传播提供依据。利用常规细菌分离培养、PCR方法、微量肉汤稀释法进行MRSA的分离鉴定、药敏试验与多位点序列分型(MLST)。结果显示:(1)10省4755份样品共分离到1442株金黄色葡萄球菌,其中239株为MRSA,包括猪源MRSA 203株(18.78%),牛源27株(14.44%),鸡源9株(5.17%)。(2)多省猪源MRSA对青霉素耐药率100%,对氟喹诺酮类和头孢菌素类耐药率在80%以上,其余药物均在20%~60%;牛源/鸡源MRSA对青霉素、红霉素、克林霉素和头孢菌素类耐药率100%,但普遍对磺胺类敏感,牛源未检测到利奈唑胺耐药菌株。(3)各省份ST型分布存在差异,检测的179株MRSA共分为4个序列型,ST 9最多,在3种动物源中均有分布,其次是ST 59;ST 398和ST 1376仅出现在猪源菌株。(4)ST 9和ST 1376的亲缘关系在85%左右,同一地区、同一ST型菌株可产生不同的耐药谱。表明MRSA在我国不同地区不同动物中均有分布,猪源样品检出率最高,ST 9仍为优势型别;不同来源MRSA分离菌株存在耐药差异,且菌株基因型和耐药表型之间无相关性。To study the distribution characteristics and drug resistance of methicillin-resistant Staphylococcus aureus(MRSA)from different sources in China.Strains were isolated and identified by conventional bacterial isolation method and PCR technology.And then,micro broth dilution method was used to detect drug-resistance phenotype,and multi-locus sequence syping(MLST)technique was applied to carry out molecular type.Results showed that a total of 1442 Staphylococcus aureus(S.aureus)were isolated,239 of which were MRSA,including 203(18.78%)swine MRSA,27(14.44%)bovine MRSA,and 9(5.17%)chicken MRSA.The resistance rate of swine MRSA to penicillin was 100%,to fluoroquinolones and cephalosporins were more than 80%,and to other drugs were between 20%and 60%in many provinces.The resistance rate of bovine/chicken MRSA to penicillin,erythromycin,clindamycin and cephalosporins were 100%,which were sensitive to sulfonamides.There was no resistant bovine strain to linezolid detected.Four STs were obtained from 179 strains tested by MLST.ST 9 was the most popular and distributed in all three animal sources,then followed by ST 59.ST 398 and ST 1376 were distributed only in swine strains.The relationship between ST 9 and ST 1376 was about 85%by cluster analysis.MRSA distributed in different animals and different regions in China,and the detection rate from pigs was the highest,while ST 9 was still the dominant type.Differences in drug resistance existed between different sources,and no correlation was observed between genotype and drug resistance.

关 键 词：耐甲氧西林金黄色葡萄球菌 分离鉴定 耐药性 多位点序列分型 

分 类 号：R378.1[医药卫生—病原生物学]