西瓦克颗粒指纹图谱及抗氧化活性研究  

Study on the fingerprint and antioxidant activity of Xwak Granules

作　　者：陈丽[1,2] 刘柳 马庆苓 希尔艾力·吐尔逊[3] 吴涛 信学雷[1] 阿吉艾克拜尔·艾萨[1] CHEN Li;LIU Liu;MA Qingling;Xirali Tursun;WU Tao;XIN Xuelei;Haji Akber Aisa(Key Lab of Chemistry of Plant Resources in Arid Regions,the Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Xinjiang Uygur Autonomous Region,Urumqi 830011,China;Graduate School,University of Chinese Academy of Sciences,Beijing 100049,China;Key Laboratory of Uighur Medical Prescription and Pharmacology,Uighur Medical Research Institute,Xinjiang Uygur Autonomous Region,Urumqi 830049,China)

机构地区：[1]中国科学院新疆理化技术研究所干旱区植物资源化学重点实验室,新疆乌鲁木齐830011 [2]中国科学院大学研究生院,北京100049 [3]新疆维吾尔自治区维吾尔医药研究所维吾尔医方剂学重点实验室,新疆乌鲁木齐830049

出　　处：《中国医药导报》2021年第16期14-18,F0003,共6页China Medical Herald

基　　金：国家自然科学基金新疆联合基金重点项目(U1703235);中国科学院“西部之光”人才培养引进计划项目(2019-XBQNXZ-B-012)。

摘　　要：目的建立西瓦克颗粒高效液相色谱和薄层色谱指纹图谱,同时测定5个指标成分的含量,并初步考察西瓦克颗粒的抗氧化活性。方法高效液相色谱采用InertSustain C18色谱柱,流动相为乙腈-0.2%磷酸,柱温30℃,分段检测波长为245、260、300、320、350、360 nm,流速1 mL/min,进样体积10μL;采用“中药色谱指纹图谱相似度评价系统(2004A版)”进行相似度分析;建立同时测定5个特征化合物的含量测定方法;采用薄层色谱-生物自显影法考察其抗氧化活性。结果10个不同批次的西瓦克颗粒指纹图谱相似度均≥0.811,确定共有峰36个,对其中5个特征峰进行了归属,分别为没食子酸、3,5-O-二咖啡酰奎宁酸、异槲皮苷、4,5-O-二咖啡酰奎宁酸、一枝蒿酮酸;西瓦克颗粒含量测定方法学考察线性系数均≥0.9981,特征峰精密度、重复性、稳定性和加样回收率的相对标准差均<3%,同时测定了这5个指标成分的含量;西瓦克颗粒对2,2’-氨基-二(3-乙基-苯并噻唑啉-6-磺酸)(ABTS)和1,1’-二苯基-2-苦肼基(DPPH)具有一定的清除作用。结论所建立的指纹图谱方法准确、高效、重复性好,可用于西瓦克颗粒的质量控制,可同时测定5种成分的含量。西瓦克颗粒中含有许多具有清除ABTS和DPPH自由基作用的成分,具有一定的抗氧化活性。Objective To establish the high performance liquid chromatography and thin layer chromatography fingerprints of Xwak Granules,simultaneously determine the contents of five index components,and preliminarily investriage the antioxidant activity of Xwak Granules.Methods High performance liquid chromatography was performed on InertSustain C18 column.The mobile phase was acetonitrile-0.2%phosphoric acid,and the column temperature was 30℃.The segmented detection wavelengths were 245,260,300,320,350 and 360 nm,the flow rate was 1 mL/min,and the injection volume was 10μL.The similarity analysis was carried out by the“similarity evaluation system for traditional Chinese medicine chromatographic fingerprint(2004A edition)”.A method for simultaneous determination of five characteristic compounds was established.The antioxidant activity was investigated by thin layer chromatography-bioautoradiography method.Results The similarity of the fingerprints of ten different batches of Xwak Granules was higher than 0.811,36 common peaks were identified.Five characteristic peaks were identified as gallic acid,3,5-O-dicaffeoylquinic acid,isoquercitrin,4,5-O-dicaffeoylquinic acid and rupestonic acid.The linear coefficients of Xwak Granules content determination method were more than 0.9981.The relative standard deviations of precision,reproducibility,stability and recovery of characteristic peaks were all lower than 3%.Meanwhile,the contents of these five index components were determined.Xwak Granules had certain scavenging effects on 2,2’-amino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS)and 1,1-diphenyl-2-picrylhydrazyl(DPPH).Conclusion The established fingerprint method is accurate,efficient and reproducible.It can be used for the quality control of Xwak Granules and the content of five components can be determined simultaneously.Xwak Granules contain many components which can scavenging ABTS and DPPH free radicals and have certain antioxidant activity.

关 键 词：西瓦克颗粒 指纹图谱 相似度分析 含量测定 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]