塔玛亚历山大藻产麻痹性贝类毒素能力的研究  

On paralytic shellfish poisoning producing capacity of Alexandrium tamarense

作　　者：汪宇 张海燕[1] 隋延鸣[1] 汤云瑜 孔聪[1] 王媛[1] 刘淑晗 蔡友琼[1] 沈晓盛[1] WANG Yu;ZHANG Haiyan;SUI Yanming;TANG Yunyu;KONG Cong;WANG Yuan;LIU Shuhan;CAI Youqiong;SHEN Xiaosheng(East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学食品学院,上海201306

出　　处：《海洋渔业》2021年第3期359-369,共11页Marine Fisheries

基　　金：中国水产科学研究院基本科研业务费(2017HY-YJ0202);中央级公益性科研院所基本科研业务费(2185-2018);国家自然科学基金(31471672);上海市自然科学基金(17ZR1439400)。

摘　　要：为探究塔玛亚历山大藻(Alexandrium tamarense)产麻痹性贝类毒素(paralytic shellfish poisoning,PSP)的能力,通过设置不同空间效应、盐度和营养素浓度对塔玛亚历山大藻进行培养,分析不同培养条件下塔玛亚历山大藻产麻痹性贝类毒素的能力。结果表明:1)塔玛亚历山大藻中至少含有13种PSP组分,其中主要成分为GTX1、GTX4、GTX5和C1、C2,占总毒素的97.2%。当藻细胞数量低于4×10^(3)个时,13种组分均不能定量检出;当藻细胞数量在4×10^(3)~5.9×10^(6)个时,13种组分不能完全定量检出;当藻细胞数高于5.9×10^(6)个时,13种组分均能够检出。2)不同条件下塔玛亚历山大藻产PSP能力比较,盐度25、35培养组显著高于盐度15培养组,1 L培养组显著高于0.1 L培养组,寡营养培养组显著高于富营养组。研究结果表明,塔玛亚历山大藻具有丰富的毒素谱,且分别在盐度25、1 L培养液和1/4倍营养素浓度下具有最佳产毒能力。In order to explore the capacity of paralytic shellfish poisoning(PSP)production in Alexandrium tamarense,the algae strain was cultivated in different space volumes,salinities and nutrient concentrations with controlling other conditions unchanged.After culturing for a period of time,a certain number of algal cells were collected by centrifugation to analyze and calculate the toxin content and profiles by liquid chromatography tandem-mass spectrometry(LC-MS/MS).Results showed that:1)A.tamarense could produce at least 13 PSP components,of which the main components were GTX1,GTX4,GTX5,C1 and C2,accounting for 97.2%of the total toxin content,and the content of GTX1 was as high as 40%.At the same time,there was a significant difference in the minimum number of A.tamarense cells required for extraction and the quantitative detection of each component.For example,when the number of algal cells used to extract and analyze toxin profiles was less than 4×10^(3),none of the 13 PSP components could be quantitatively detected.When the number of algae cells used to extract and analyze toxin profiles was between 4×10^(3) and 5.9×10^(6),only part of the toxin components could be quantitatively detected.All these 13 PSP components could be quantified only when the number of algae cells used to extract and analyze toxin profiles was higher than 5.9×10^(6).2)The PSP producing capacity of A.tamarense cultured in salinity 25 and 35 was significantly higher than that cultured in salinity 15,the PSP producing capacity of A.tamarense cultured in the flask of 1 L was significantly higher than that cultured in the flask of 0.1 L,and cultured in the oligotrophic group was significantly higher than that cultured in the eutrophic group.From these results,we could know that A.tamarense had a rich toxin profile,and contained at least 13 PSP toxin components with known structures.A.tamarense could be used for basic research of PSP toxin,and it was also the best theoretical algae strain for separating and extracting GTX1.It showed stronger

关 键 词：塔玛亚历山大藻 麻痹性贝类毒素 产毒能力 

分 类 号：Q945.78[生物学—植物学]