肝纤维化大鼠肝郁病证结合模型的建立与评价  

Establishment and Evaluation of Liver-Depression Disease and Syndrome Combination Model in Rats with Liver Fibrosis

作　　者：李鑫[1] 王权 陈思源[2] 张逢 余曦明[2] LI Xin;WANG Quan;CHEN Si-yuan;ZHANG Feng;YU Xi-ming(The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410000,China;Hunan University of Traditional Chinese Medicine,Changsha 410028,China)

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410000 [2]湖南中医药大学,湖南长沙410028

出　　处：《云南中医中药杂志》2021年第6期66-69,共4页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：湖南省大学生研究性学习和创新性实验计划(湘教通〔2018〕147号)。

摘　　要：目的通过构建肝纤维化大鼠肝郁病证结合的模型,为相关研究提供模型参考依据和临床治疗提供诊疗思路。方法将30只SD大鼠随机分成2组,即空白组和模型组,每组15只。模型组大鼠每天定期皮下注射四氯化碳(carbon tetrachloride,CCL4)+橄榄油,自第5周起每日加用夹尾进行刺激,每次30min,3次/天;空白组大鼠每天定期皮下注射橄榄油,2组均连续给药8周。实验结束后记录动物和肝脏重量,收集肝脏组织制作石蜡切片染色后观察其病理变化,用ELISA法检测血清中AST和ALT相关活力指标。结果(1)与空白组比较,模型组肝脏重量明显增加(P<0.05),血清中AST和ALT的活力显著升高(P<0.05或P<0.01)。(2)形态学结果显示:模型组大鼠肝脏出现了典型的肝纤维化病理改变,主要表现为肝组织内纤维结缔组织增生,脂肪空泡形成及相关炎性细胞浸润等。结论采用此种方法造模可升高大鼠血清的AST和ALT活力,肝脏呈纤维化改变,成功建立了肝纤维化大鼠模型,是较为理想的造模方法。Objective:To provide a model reference basis and clinical treatment ideas for related research by constructing a model combining liver depression and syndromes in rats with liver fibrosis.Methods:30 SD rats were randomly divided into 2 groups,namely the blank group and the model group,15 rats per group.The rats in the model group were given regular subcutaneous injections of carbon tetrachloride(CCL4)+olive oil every day,and starting from the 5th week,the tail clamp was added every day for stimulation,30 minutes each time,3 times a day.The rats in the blank group were given subcutaneous injection of olive oil every day.Both groups were given continuous administration for 8 weeks.After the experiment,the weight of the animals and liver was recorded.The liver tissues were collected to make paraffin sections and stained to observe the pathological changes.ELISA method was used to detect the AST and ALT related vitality indicators in the serum.Results:(1)Compared with that of the blank group,the liver weight of the model group was significantly increased(P<0.05),and the activity of AST and ALT in the serum was significantly increased(P<0.05 or P<0.01).(2)The morphological results showed that the liver of the model group showed typical pathological changes of liver fibrosis,which were mainly manifested as the hyperplasia of fibrous connective tissue in the liver tissue,the formation of fatty vacuoles and the infiltration of related inflammatory cells.Conclusion:Modeling with this method can increase the activity of AST and ALT in rat serum,and the liver shows fibrotic changes.The successful establishment of a rat model of liver fibrosis is an ideal method for modeling.

关 键 词：肝纤维化 大鼠 模型 肝功能 

分 类 号：R575[医药卫生—消化系统]