MiR-34a可双向调控感染及非感染状态成骨样细胞的成骨分化  

Mutual Regulation of mi R-34a on osteogenic differentiationn of osteoblast-like cells under Infectious and Non-Infectious Status

作　　者：高涵 林崇韬[2] GAO Han;LIN Chongtao(Zhuhai Stomatological Hospital,Zhuhai 519000)

机构地区：[1]广东省珠海市口腔医院,519000 [2]吉林大学口腔医院牙周科

出　　处：《现代口腔医学杂志》2021年第3期145-148,共4页Journal of Modern Stomatology

基　　金：国家自然科学基金面上项目资助课题(81371153);吉林省科技厅自然科学基金面上项目资助课题(20150101173JC)。

摘　　要：目的将微小RNA-34a(miR-34a)转染至健康及炎症状态下成骨样细胞,检测其对细胞成骨分化能力的影响。方法取人成骨样细胞(MG63)为实验细胞,将miR-34a拟态物转染至细胞并用实时定量PCR法(RTPCR)及荧光显微镜检测转染效率。RT-PCR检测受牙龈卟啉单胞菌(Pg)感染的MG63细胞内miR-34a表达量变化及转染miR-34a后健康或炎症状态下MG63细胞中成骨相关因子Ⅰ型胶原(COLⅠ)、Runt相关转录因子2(Runx2)及Osterix成骨相关转录因子(SP7)表达量变化。结果 MiR-34a有效转染至MG63细胞内;miR-34a可促进MG63细胞内COLⅠ、Runx2及SP7基因表达;炎症状态下MG63细胞内miR-34a表达量下降;miR-34a降低感染状态下MG63细胞内COLⅠ、Runx2及SP7基因表达量。结论证实miR-34a促进MG63细胞成骨分化,当细胞处于感染状态时,其抑制细胞成骨分化。Objective To detect the effects of miR-34a on osteogenic differentiation of osteoblast-like cells in healthy and inflammatory conditions. Methods MG63 were selected as experimental cells. MiR-34a mimics were transfected into MG63 and real-time PCR and Fluorescence microscopy were used to detect the efficiency miR-34 a transfection. MG63 was infected with Pg, then the expression of miR-34a in infected cells and the expression of COL Ⅰ,Runx2 and SP7 at the level of mRNA in MG63 under healthy and inflammatory conditions were detected by real-time PCR. Results miR-34a could be effectively transfected into MG63;miR-34a up-regulated the expression of COL Ⅰ,Runx2 and SP7 at the level of mRNA in MG63;The expression of miR-34 a in infected MG63 was decreased;miR-34a down-regulated the expression of COLⅠ, Runx2 and SP7 at the level of mRNA in infected MG63. Conclusion It was confirmed that miR-34 a can promote osteogenic differentiation of MG63,whereas it inhibit osteogenic differentiation of the cells in infected condition.

关 键 词：人成骨样细胞 MIR-34A 成骨相关因子 

分 类 号：R780.2[医药卫生—口腔医学]