miR-132通过靶向KLF7抑制鼻咽癌细胞增殖和迁移  

miR-132 inhibits proliferation and migration of nasopharyngeal carcinoma cells by targeting KLF7

作　　者：张可[1] 姜岚[1] 僧东杰 朱卿文 ZHANG Ke;JIANG Lan;SENG Dong-jie;ZHU Qing-wen(Department of Otolaryngological,Children's Hospital Affiliated of Zhengzhou University&Henan Children's Hospital&Zhengzhou Children's Hospital,Zhengzhou 450018,China)

机构地区：[1]郑州大学附属儿童医院、河南省儿童医院、郑州儿童医院耳鼻喉科,450018

出　　处：《天津医药》2021年第6期577-582,共6页Tianjin Medical Journal

基　　金：2019年河南省医学科技攻关计划联合共建项目(编号LHGJ20190954)。

摘　　要：目的探讨miR-132靶向负调节Kruppel样因子7(KLF7)表达对鼻咽癌(NPC)细胞增殖和迁移的影响。方法将NPC细胞CNE-2Z分组:空白组、NC组、miR-132 mimics组、KLF7-OE组和miR-132 mimics+KLF7-OE组。荧光定量PCR(qPCR)检测NPC组织和细胞及正常鼻咽组织和细胞中miR-132表达,Western blot检测KLF7蛋白表达。TargetScan网站预测及双荧光素酶检测验证KLF7与miR-132的靶向关系。CCK-8实验和Transwell实验检测CNE-2Z细胞增殖、迁移和侵袭能力,裸鼠成瘤实验、免疫组化法检测CNE-2Z细胞体内生长、瘤内血管情况。结果鼻咽癌组织较正常鼻咽组织miR-132表达水平降低,KLF7蛋白表达增加;鼻咽癌CNE-2Z细胞较正常鼻咽上皮细胞NP69 miR-132表达水平降低,KLF7蛋白表达增加(P<0.05)。TargetScan预测及双荧光素酶检测显示,KLF7是miR-132的靶基因。相比空白组、NC组,miR-132 mimics组CNE-2Z细胞中KLF7蛋白表达水平,36、48、60 h细胞增殖活力,迁移和侵袭数量,肿瘤体积及微血管密度(MVD)显著降低(P<0.05),KLF7-OE组结果均相反(P<0.05)。相比miR-132 mimics组,miR-132 mimics+KLF7-OE组KLF7蛋白表达水平,36、48、60 h细胞增殖活力,迁移和侵袭数量、肿瘤体积及MVD显著升高(P<0.05)。结论miR-132可通过负调控KLF7表达,影响NPC细胞的增殖、迁移及侵袭。Objective To investigate the effects of miR-132 on the proliferation and migration of nasopharyngeal carcinoma(NPC)cells by negatively targeting the expression of Kruppel-like factor 7(KLF7).Methods NPC CNE-2Z cells were divided into blank group,NC group,miR-132 mimics group,KLF7-OE group and miR-132 mimics+KLF7-OE group.The expressions of miR-132 in NPC tissues and cells and normal nasopharyngeal tissues and cells were detected by fluorescence quantitative PCR(qPCR).The expression level of KLF7 protein was detected by Western blot assay.The targeting relationship between KLF7 and miR-132 was predicted and verified by TargetScan website and dual luciferase detection.CCK-8 test and Transwell test were used to detect the proliferation,migration and invasion of CNE-2Z cells.The tumor formation test in nude mice and immunohistochemistry were used to detect the growth of CNE-2Z cells in vivo and the blood vessels in tumor.Results The expression level of miR-132 was decreased in nasopharyngeal carcinoma tissue than that in normal nasopharyngeal tissue,and the expression of KLF7 protein was increased.The expression level of miR-132 was decreased in nasopharyngeal carcinoma CNE-2Z cells than that in normal nasopharyngeal epithelial cells NP69,and the expression of KLF7 protein was increased(P<0.05).TargetScan prediction and dual luciferase detection showed that KLF7 was the target gene of miR-132.Compared with those in the blank group and NC group,the expression of KLF7 protein,cell proliferation activity at 36,48 and 60 h,numbers of migration and invasion,tumor volume and microvessel density(MVD)in CNE-2Z cells were significantly decreased in miR-132 mimics group(P<0.05),while the results were oppositive in KLF7-OE group(P<0.05).Compared with those in miR-132 mimics group,the expression level of KLF7 protein,cell proliferation activity at 36,48 and 60 h,numbers of migration and invasion,tumor volume and MVD were significantly increased in miR132 mimics+KLF7-OE group(P<0.05).Conclusion miR-132 can affect the proliferatio

关 键 词：鼻咽肿瘤 细胞增殖 细胞运动 Kruppel样转录因子类 微RNAS miR-132 Kruppel样因子7 

分 类 号：R739.6[医药卫生—肿瘤]