冷杉属叶绿体基因组比较与系统发育分析  

Comparative Study on Chloroplast Genome and Phylogenetic Analysis of Abies

作　　者：李佳 赵梦瑶 张朝霞 相世英 郑宏春 贺游利 LI Jia;ZHAO Mengyao;ZHANG Chaoxia;XIANG Shiying;ZHENG Hongchun;HE Youli(College of life science and food engineering,Shaanxi Xueqian Normal University,Shaanxi Xi'an 710100)

机构地区：[1]陕西学前师范学院生命科学与食品工程学院,陕西西安710100

出　　处：《生物化工》2021年第3期56-60,共5页Biological Chemical Engineering

基　　金：陕西学前师范学院省级大学生创新创业训练计划项目(7354);国家级大学生创新创业训练计划项目(201914390020);陕西省教育厅科研计划项目(19Jk0211)。

摘　　要：以冷杉属10个物种的叶绿体全基因组为对象,分析其基因含量、重复序列等,重建冷杉属物种之间的系统进化关系。分析结果显示:冷杉属植物10个物种的叶绿体基因组大小在120057~121799 bp,基因个数在113~120,蛋白编码基因个数在71~74;冷杉属的10个物种中每一个物种的简单重复序列个数和分布情况存在差异,这可能是冷杉属植物在进化过程中不同物种的基因序列缺失、突变等原因造成;最大似然法与邻接法的系统发育树结果分为三支,南方红豆杉、西藏红豆杉、日本柳杉为一支,巴西南洋杉为一支,冷杉属10个物种为一支。在冷杉属,邻接法结果显示梵净山冷杉单独分为一支,其余9个物种分为一支;在最大似然法中欧洲冷杉与梵净山冷杉聚为一支,其余8个物种聚在一支。In this study,the gene content,repeat sequence of 10 Abies species chloroplast genome were analyzed.The phylogenetic relationship between 10 Abies species were also reconstructed.The results showed that:The complete chloroplasts genome size of Abies is between 120057~121799 bp,the number of genes is between 113~120,and the number of protein coding genes is between 71 and 74.There are differences in the number and distribution of simple sequence repeat in each of the 10 species,which may be caused by sequence deletion and mutation.The phylogenetic trees constructed by maximum likelihood and neighbor-joining method divided 10 Abies species and four outgroups into three branches.The first branch including Taxus chinensis,Taxus wallichiana and Cryptomeria japanica;the second including Araucaria angustifolia,and 10 Abies species formed the third group.Within Abies,the Abies fanjingshanensis formed one branch while other nine species cluster together using Neighbor-joining method.The Maximum Likelihood tree showed that Abies alba and Abies fanjingshanensis grouped together,while the other eight species consist a monophyletic group.

关 键 词：冷杉属 叶绿体基因组 系统发育 

分 类 号：Q943.2[生物学—植物学]