Stat3抑制剂Stattic抑制人舌鳞癌细胞增殖的机制研究  

A study of the effects of Stattic on the proliferation of human tongue squamous cell carcinoma cells

作　　者：赵卿 张晓月 刘海丹[3] 李明[1,2] ZHAO Qing;ZHANG Xiao-yue;LIU Hai-dan;LI Ming(Hunan University of Traditional Chinese Medicine,Hunan Changsha 410208,China;Changsha Stomatological Hospital,Hunan Changsha 410004,China;The 2nd Xiangya Hospital of Central South University,Hunan Changsha 410011,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]长沙市口腔医院,湖南长沙410004 [3]中南大学湘雅二医院,湖南长沙410011

出　　处：《临床口腔医学杂志》2021年第5期272-275,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金(项目编号:81904262);湖南省自然科学基金青年科学基金(项目编号:2019JJ50682);湖南省卫生计生委科研基金(项目编号:202108051626)。

摘　　要：目的:观察Stat3小分子抑制剂Stattic对人舌鳞癌细胞的增殖抑制作用。方法:用不同浓度Stattic处理人舌鳞癌细胞SCC-4与SCC-25,采用CCK-8试剂盒检测Stattic对两株细胞增殖的影响。采用Western Blot方法检测Stat3 Ser727,Stat3 Tyr705磷酸化水平以及Rictor蛋白表达水平;凋亡调控分子Bcl-2,Mcl-1,Bax,Survivin表达水平。结果:Stattic呈浓度依赖性抑制人舌鳞癌细胞的增殖(P<0.05),经Stattic干预的人舌鳞癌细胞中Stat3 Ser727、Stat3 Tyr705磷酸化水平降低,Rictor表达降低。Stattic诱导人舌鳞癌细胞Caspase3和PARP剪切体表达上调,诱导抗凋亡蛋白Survivin表达降低,而抗凋亡蛋白Bcl-2,Mcl-1表达水平无明显变化,促凋亡蛋白Bax表达水平无明显变化。结论:Stattic对人舌鳞癌细胞增殖的抑制作用,可能与降低Stat3 Ser727和Stat3 Tyr705磷酸化水平、降低Rictor及Survivin蛋白表达有关。Objective:To observe the inhibitory effects of signal transducer and activator of transcription 3(Stat3)inhibitor Stattic on the viability of human tongue squamous cell carcinoma cells.Methods:Human tongue cancer cells SCC-4 and SCC-25 were treated with various concentrations of Stattic,the inhibitory effect of Stattic on cell viability of two cell lines were detected by CCK-8 assay.The apoptosis-regulated proteins,including Bcl-2,Mcl-1,Bax,Survivn,as well as Stat3 phosphorylation levels at Ser727 and Tyr705,Rictor protein were detected by Western Blot.Results:Stattic inhibited the proliferation of human tongue squamous cell carcinoma cells in a concentration dependent manner(P<0.05).The level of phosphorylation in human tongue squamous cells Stat3 Ser727 Stattic intervention decreased,Stat3 Tyr705 phosphorylation levels decreased after 10μmol/L of Stattic intervention,Rictor expression decreased.Stattic induce upregulation of Caspase3 and PARP shear body expression in human tongue squamous cell carcinoma cells and induce decreased expression of anti-apoptotic protein Survivin,yet the expression level of anti-apoptotic protein Bcl-2,Mcl-1 had no obvious change,the expression level of pro-apoptotic protein Bax had no obvious change.Conclusion:Stattic inhibits the viability of human tongue squamous cell carcinoma cells through reducing phosphorylation level of Stat3,Survivin and Rictor protein expression.

关 键 词：人舌鳞癌细胞 Stattic STAT3 SURVIVIN RICTOR 

分 类 号：R739.8[医药卫生—肿瘤] Q813.1[医药卫生—临床医学]