丙泊酚对胃癌细胞凋亡时GRP78-ATF4-CHOP信号通路的影响  

Effects of propofol on GRP78-ATF4-CHOP signaling pathway during apoptosis in gastric cancer cells

作　　者：张苗[1] 岳维[2] 顾晓东 Zhang Miao;Yue Wei;Gu Xiaodong(College of Anesthesiology,Shanxi Medical University,Taiyuan 030001,China;Department of Anesthesiology,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China;Department of Orthopaedics,Shanxi Bethune Hospital,Taiyuan 030001,China)

机构地区：[1]山西医科大学麻醉学院,太原030001 [2]山西医科大学第二医院麻醉科,太原030001 [3]山西白求恩医院骨科,太原030001

出　　处：《中华麻醉学杂志》2021年第2期239-241,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金青年项目(34100940);教育部高等学校博士点专项科研基金(20131417120001);山西省教育厅高等学校科技创新项目(2013120)。

摘　　要：目的评价丙泊酚对胃癌细胞凋亡时葡萄糖调节蛋白78(GRP78)-活化转录因子4(ATF4)-C/EBP同源蛋白(CHOP)信号通路的影响。方法传代培养至对数期的MGC-803细胞,采用随机数字表法分为2组(n=15):对照组(C组)和丙泊酚组(P组)。C组细胞在37℃、5%CO2条件下正常培养;P组细胞待贴壁生长至70%~90%时,加入丙泊酚,终浓度为5μg/ml,24 h时采用流式细胞术检测细胞凋亡率,qPCR法检测GRP78、ATF4和CHOP的mRNA表达,Western blot法检测GRP78、ATF4和CHOP的表达。结果与C组比较,P组细胞凋亡率升高,GRP78、ATF4、CHOP及其mRNA表达上调(P<0.05)。结论丙泊酚促进胃癌细胞凋亡的机制与激活GRP78-ATF4-CHOP信号通路有关。Objective To evaluate the effects of propofol on glucose-regulated protein 78(GRP78)-activating transcription factor-4(ATF4)-CCAAT/enhancer binding protein(C/EBP)homologous protein(CHOP)signaling pathway during apoptosis in gastric cancer cells.Methods MGC-803 cells were cultured to the logarithmic phase and divided into 2 groups(n=15 each)using the random number table method:control group(group C)and propofol group(group P).The cells in group C were cultured under normal conditions of 37℃and 5%CO2.In group P,propofol(final concentration 5μg/ml)was added when the cells were attached to the wall and grown to 70%-90%confluence.At 24 h after propofol was added,the apoptosis rate of cells was determined by flow cytometry,the expression of GRP78,ATF4 and CHOP mRNA was detected by quantitative polymerase chain reaction,and the expression of GRP78,ATF4 and CHOP was determined by Wester blot,respectively.Results Compared with group C,the apoptosis rate was significantly increased,and expression of GRP78,ATF4,CHOP and its mRNA was up-regulated in group P(P<0.05).Conclusion The mechanism by which propofol accelerates apoptosis is related to activating GRP78-ATF4-CHOP signaling pathway in gastric cancer cells.

关 键 词：二异丙酚 胃肿瘤 细胞凋亡 热休克蛋白质类 转录因子 内质网应激 

分 类 号：R735.2[医药卫生—肿瘤]