敲低干细胞关键转录因子FoxD3对胃癌细胞SGC-7901的影响  

Effect of knockdown of key transcription factor FoxD3 on gastric cancer cell line SGC-7901

作　　者：刘世光 田伶伶 史冬梅 王聪[2] 刘聪[3] LIU Shi-guang;TIAN Ling-ling;SHI Dong-mei;WANG Cong;LIU Cong(Department of Medical Oncology,Liaoyang Central Hospital,Liaoyang Liaoning 111000,China;Department of Medical Oncology,Liaoning Cancer Hospital,Shenyang Liaoning 110000,China;Department of Medical Oncology,Tieling Central Hospital,Tieling Liaoning 112000,China)

机构地区：[1]辽阳市中心医院肿瘤内科,辽宁辽阳111000 [2]辽宁省肿瘤医院肿瘤内科,辽宁沈阳110000 [3]铁岭市中心医院肿瘤内科,辽宁铁岭112000

出　　处：《局解手术学杂志》2021年第7期564-568,共5页Journal of Regional Anatomy and Operative Surgery

基　　金：辽宁省自然科学基金(20180550046)。

摘　　要：目的探究shRNA FoxD3对胃癌细胞SGC-7901生长、运动及上皮间质转化的影响。方法将shRNA FoxD3载体和空质粒载体转染至SGC-7901细胞,根据处理方式不同,将细胞分为Control组、shRNA-NC组和FoxD3-shRNA2组。克隆形成实验检测细胞克隆形成率;Transwell检测侵袭细胞数;划痕实验检测细胞划痕愈合率;显微镜观察上皮间质转化形态学变化;Western blot检测Ki67、增殖细胞核抗原(PCNA)、上皮钙黏蛋白(E-cadherin)、神经钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)蛋白表达水平。结果与Control组比较,FoxD3-shRNA2组克隆形成率降低(P<0.05),Ki67、PCNA蛋白表达降低(P<0.05),侵袭细胞数减少(P<0.05),划痕愈合率降低(P<0.05),上皮间质转化受到抑制,E-cadherin蛋白表达升高(P<0.05),N-cadherin和Vimentin蛋白表达降低(P<0.05)。结论shRNA FoxD3可抑制SGC-7901细胞的增殖、侵袭、迁移和上皮间质转化。Objective To investigate the effect of shRNA FoxD3 on the growth,motility and epithelial-mesenchymal transformation of gastric cancer cell line SGC-7901.Methods The shRNA FoxD3 vector and empty plasmid vector were transfected into SGC-7901 cells,and the cells were divided into the Control group,the shRNA-NC group and the FoxD3-shRNA2 group according to different treatment methods.The rate of cell clone formation was detected by clonal formation assay;the number of invaded cells were detected by Transwell;the cell scratch healing rate was detected by scratch test;the morphological changes of epithelial-mesenchymal transformation was observed under microscope;the protein expression levels of Ki67,proliferating cell nuclear antigen(PCNA),E-cadherin,N-cadherin and Vimentin were detected by Western blot.Results Compared with the Control group,the FoxD3-shRNA2 group had lower rate of cell clone formation(P<0.05),lower protein expression of Ki67 and PCNA(P<0.05),less invaded cells(P<0.05),and lower scratch healing rate(P<0.05).And the epithelial-mesenchymal transformation in the FoxD3-shRNA2 group was inhibited and the protein expression of E-cadherin was increased(P<0.05),while the protein expressions of N-cadherin and Vimentin were decreased in the FoxD3-shRNA2 group compared with those in the Control group(P<0.05).Conclusion ShRNA FoxD3 can inhibit the proliferation,invasion,migration and epithelial-mesenchymal transformation of SGC-7901 cells.

关 键 词：胃癌 叉头框蛋白D3 增殖 上皮间质转化 

分 类 号：R735.2[医药卫生—肿瘤]